Results

Although the Berkeley Test Cells showed a possible increase in fluorescence when exposed to sound, further testing must be done to determine the if the parts are truly responding to sound. The data will be presented at the Jamboree. The Berkeley promoter BBa_K112402 was found to be a constitutive promoter, this promoter also shows a possible RFP upregulation when exposed to sound however further testing needs to be done to determine if it is actually audio inducible. The FSU iGEM promoters: BamE, OsmC, PspA, and ZntA also showed a possible increase in fluorescence, the data will also be presented at the Jamboree.

The BIOFAB parts also showed an incremental increase like we were expecting, however, it is interesting because the incremental increase was not in the order that we were expecting. The apFAB46 showed the lowest activity but was expected to be the highest. ApFAB90 was found to have medium expression, however, was expected to be low activity. Finally, apFAB82 showed the highest fluorescence, however, was expected to be the medium expression. Further investigation is needed to determine these unexpected results.