Revision as of 03:18, 18 October 2018

EXPERIMENTS

PROTOCOLS

Agarose Gel Extraction	Colony PCR
LB Media	DNA Purification from Enzymatic Reactions
Ligation Reactions	Ligation to Insert DNA to Vector DNA
PCR Amplification	PCR Product Purification
Phenol/Chloroform Extraction and Ethanol Precipitation	Phusion PCR Protocol
pJET Cloning	Plasmid Miniprep
Preparation of Chemically Competent Cells	Protein Overexpression
Restriction Enzyme Digest and Dephosphorylation	Transformation
Batch purification of his-tagged E.coli proteins with Ni-Sepharose	Copper binding Assay for CutA
Phage Infection of E. coli	SDS PAGE of proteins
Purification of his-tagged TruB	Expression of proteins in E. coli cells

@@ Line 159: / Line 159: @@
 <tr>
      <th><a href="https://static.igem.org/mediawiki/2018/2/24/T--Lethbridge_HS--TRUEE.pdf">Purification of his-tagged TruB</a></th>
-     <th><a href="https://static.igem.org/mediawiki/2018/a/ae/T--Lethbridge_HS--SDS.pdf">SDS PAGE of proteins</a></th>
+     <th><a href="https://static.igem.org/mediawiki/2018/8/85/T--Lethbridge_HS--ecoli.pdf">Expression of proteins in
+ E. coli cells</a></th>
    </tr>