Difference between revisions of "Team:Austin LASA/Applied Design"

Revision as of 03:21, 18 October 2018

h(g.Page, {title: 'Applied Design', prev: 'https://2018.igem.org/Team:Austin_LASA/Model', selector: [5, 2]},
 h('li', null, 'Here our team has detailed how our kit design has fit the requirements for the Special Award Applied Design criterion:'),
 h('li', null, 'In order to reduce infant mortality caused by HIV, children must be tested in infancy in order to begin treatment as soon as possible. However, current diagnostic kits are not geared towards testing infants, since many use antibodies as indicators or rely on PCR. For instance, all major rapid HIV detection tests (that take less than 20 minutes to reach a diagnosis) rely on antibody indicators. Not only rapid tests, but all FDA-approved HIV screening tests similarly rely on antibodies. These serological assays often give false positives at birth due to the shared antibodies between mother and child. Furthermore, tests utilizing PCR would only be able to be conducted at larger facilities, not rural hospitals, due to lack of equipment. This lack of resources hinders the detection process, as all samples would have to be sent to larger labs, and would result in a longer turnaround time for results. Thereby, if results are not provided in a timely fashion, infants who are HIV positive may leave the hospital without starting treatment, putting them in danger later on in their life.
'),
 h('li', null, 'Our kit relies on LAMP cellular reagents, CRISPR Cas12a system, along with a fluorophore-quencher assay in order to provide HIV detection. By using these protocols, we can bypass the normal machinery requirements in a lab (thermocyclers), and in its place, depend on basic laboratory systems to provide a diagnosis. By providing on site-detection capabilities, the virus could be detected within the minimal time frame of other nucleic acid tests while being significantly cheaper. This portion of the detection test can be done using a water bath and sterile transfer techniques alone. In this way, we are able to address the issues regarding the limited equipment in rural hospitals and the lack of time, by being able to provide results within the hospitals themselves. In doing so, the proposed kit allows for infants to be accurately testing upon birth, and proper treatment information can be provided to the family before they return home.  By integrating HIV detection into the point of care testing in many low-income regions, we are able to advance HIV detection to some of the most vulnerable communities, thereby improving the chance at treatment and recovery.

'),
 h('li', null, 'Nevertheless, there is still room for improvement as our research is not yet complete. Our project only details the detection of HIV-1 from purified samples of DNA, however, a protocol for purifying patient samples must be established to extract DNA on-site.
'),
);

@@ Line 3: / Line 3: @@
 <textarea id='page'>
 h(g.Page, {title: 'Applied Design', prev: 'https://2018.igem.org/Team:Austin_LASA/Model', selector: [5, 2]},
+ h('li', null, 'Here our team has detailed how our kit design has fit the requirements for the Special Award Applied Design criterion:'),
+ h('li', null, 'In order to reduce infant mortality caused by HIV, children must be tested in infancy in order to begin treatment as soon as possible. However, current diagnostic kits are not geared towards testing infants, since many use antibodies as indicators or rely on PCR. For instance, all major rapid HIV detection tests (that take less than 20 minutes to reach a diagnosis) rely on antibody indicators. Not only rapid tests, but all FDA-approved HIV screening tests similarly rely on antibodies. These serological assays often give false positives at birth due to the shared antibodies between mother and child. Furthermore, tests utilizing PCR would only be able to be conducted at larger facilities, not rural hospitals, due to lack of equipment. This lack of resources hinders the detection process, as all samples would have to be sent to larger labs, and would result in a longer turnaround time for results. Thereby, if results are not provided in a timely fashion, infants who are HIV positive may leave the hospital without starting treatment, putting them in danger later on in their life.
+'),
+ h('li', null, 'Our kit relies on LAMP cellular reagents, CRISPR Cas12a system, along with a fluorophore-quencher assay in order to provide HIV detection. By using these protocols, we can bypass the normal machinery requirements in a lab (thermocyclers), and in its place, depend on basic laboratory systems to provide a diagnosis. By providing on site-detection capabilities, the virus could be detected within the minimal time frame of other nucleic acid tests while being significantly cheaper. This portion of the detection test can be done using a water bath and sterile transfer techniques alone. In this way, we are able to address the issues regarding the limited equipment in rural hospitals and the lack of time, by being able to provide results within the hospitals themselves. In doing so, the proposed kit allows for infants to be accurately testing upon birth, and proper treatment information can be provided to the family before they return home.  By integrating HIV detection into the point of care testing in many low-income regions, we are able to advance HIV detection to some of the most vulnerable communities, thereby improving the chance at treatment and recovery.
+'),
+ h('li', null, 'Nevertheless, there is still room for improvement as our research is not yet complete. Our project only details the detection of HIV-1 from purified samples of DNA, however, a protocol for purifying patient samples must be established to extract DNA on-site.
+'),
 );
 </textarea>
 </html>