Difference between revisions of "Team:Imperial College/Part Collection"

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<h1> Part Collection </h1>
 
<h1> Part Collection </h1>
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<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
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<p>The PixCell library is the first ever library of electrogenetic parts. It contains 5 SoxR transcription factors and 8 pSoxS promoters capable of providing electronic gene induction. Parts were sourced from bacterial homologues or created by mutagenesis. Transcription factors and promoters were codon optimised for E. coli and promoters were engineered to be unidirectional, have upstream terminators for easy assembly, and have downstream ribozymes to reduce context dependency. All part combinations were characterised, exhibiting a range of RFU values with  a tunable orthogonal pair being identified. One mutant promoter we designed provided transcriptional repression, further increasing the complexity of electrogenetic circuits that can be constructed. All parts have been submitted in a BioBrick, BASIC and Golden Gate compatible format. Library parts were used to construct an electrogenetic biocontainment device whilst the system’s ability to sense oxidative stress offers extended-uses in various therapeutic, diagnostic and environmental applications.
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While you should put all the characterization information for your parts on the Registry, you are encouraged to explain how all your parts form a collection on this page.  
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Revision as of 03:29, 18 October 2018

Part Collection

The PixCell library is the first ever library of electrogenetic parts. It contains 5 SoxR transcription factors and 8 pSoxS promoters capable of providing electronic gene induction. Parts were sourced from bacterial homologues or created by mutagenesis. Transcription factors and promoters were codon optimised for E. coli and promoters were engineered to be unidirectional, have upstream terminators for easy assembly, and have downstream ribozymes to reduce context dependency. All part combinations were characterised, exhibiting a range of RFU values with a tunable orthogonal pair being identified. One mutant promoter we designed provided transcriptional repression, further increasing the complexity of electrogenetic circuits that can be constructed. All parts have been submitted in a BioBrick, BASIC and Golden Gate compatible format. Library parts were used to construct an electrogenetic biocontainment device whilst the system’s ability to sense oxidative stress offers extended-uses in various therapeutic, diagnostic and environmental applications.

Note

This page should list all the parts in the collection your team made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.

Best Part Collection Special Prize

To be eligible for this award, these parts must adhere to Registry sample submission guidelines and have been sent to the Registry of Standard Biological Parts. If you have a collection of parts you wish to nominate your team for this special prize, make sure you add your part numbers to your judging form and delete the box at the top of this page.