Revision as of 03:39, 18 October 2018

■Instructors

• Dr. Makoto Kitabatake (Institute for Frontier Life and Medical Sciences, Kyoto University) helped us all through our project, taught the way of management of a team, provided us with a laboratory , and gave us professional advice as well as training us on the experiment of S. cerevisiae. In addition, he helped collect and analysis experiment data, and reform our wiki writing. He also gave us safety education and ethics education.
• Dr. Knut Woltjen (Center of iPS Cell Research and Application, and Hakubi Center for Advanced Research, Kyoto University) helped us all through our project, and gave us professional advice on cloning. Moreover, he supported our sequencing.

■Lab support

• Dr. Dan Ohtan Wang (Institute for Integrated Cell-Material Sciences, iCeMS, Kyoto University) provided us with a laboratory and some reagents.
• Dr. Fujiwara (Institute for Integrated Cell-Material Sciences, iCeMS, Kyoto University) taught us usage of experiment equipment and gave us professional advice on cloning.
• Prof. Motohashi(Division of Life Sciences, Kyoto Sangyo University) taught us the detailed protocol and technique of SLiCE method, and gave us advice on our cloning. Moreover, he provided us with SLiCE solution and JM109.

■Fundraising help and advice

• COSMO BIO provided us with reagents and kits, free of charge.
• Promega Corporation provided us with regents and kits, free of charge.

■Project support and advice

• Prof. Uozumi (Department of Biomolecular Engineering Graduate School of Engineering, Tohoku University) gave us a yeast mutant strain and AtHKT1 expression plasmid, and advised our project design.
• Mr. Suguru E. Tanaka (Graduate School of Agricultural and Life Sciences, The University of Tokyo) trained us on culturing B. xylophilus, and provided professional advice on the experiments
• Dr. Ishida (People’s Institute of Environment) taught us seriousness of salt damage.
• Dr. Funakawa (Kyoto University, Graduate School of Global Environmental Studies) gave us hints to apply our device.
• Kinki Agricultural Bureau taught us agriculture and salt damage in Japan.
• Dr.Fujimaki (Tottori University, Arid Land Research Center) taught us about arid land drainage.
• Mr.Onishi(Japan International Research Center for Agricultural Sciences) taught us current situation of desalination works.
• Mr. Watnabe(Yamasa Soy Sauce) taught us the experimental method of soy sauce yeast and introduced us a few soy sauce yeasts.
• Dr. Inoue(Graduate School of Agriculture, Kyoto Uniersity) taught us the method to fix yeasts in column.

■Lab support

• Prof. Teiji Sota (Graduate School of Science, Kyoto University) helped us with the usage of laboratory, provided us with a laboratory, and let us use -80℃ freezer, the microscopy and Nanodrop.


• Dr. Shuhei Nasuda (Graduate School of Agriculture, Kyoto University) provided us with a laboratory and a meeting room.

■Difficult technique support

• Dr. Kobayashi (Department of Agriculture, Kyoto University) advised our project and proposed and showed us a boil method.


• Dr. Ochiai (Department of Agriculture, Kyoto University) analyzed sodium ion concentration of our samples, and mentored the analysis.
• Ms. Nakamura (Center of iPS Cell Research and Application, and Hakubi Center for Advanced Research, Kyoto University) sequenced our plasmid samples.

■Thanks and acknowledgements for all other people involved in helping make a successful iGEM team

• Mr. Sora Matsumoto (Graduate of Life Science, Kyoto University) helped us our Wiki creation and advised our project.
• Mr. Tetsu Sugimoto (Undergraduate of Engineering, Kyoto University) advised our modeling.
• Mr. Hideki Kaneko (Undergraduate of Agriculture, Kyoto University) helped the improvement of KELN by coding.
• Mr. Daiya Ohara (Undergraduate of Agriculture, Kyoto University) advised our cloning plan, helped modeling and supported us throughout our project.