Difference between revisions of "Team:Tec-Monterrey/Demonstrate"

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         <div class="body-title">Overview</div>
 
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         The complex of Cas1Cas2 proteins inserted our sequence of interest in the E.Coli BL21 sistem's. The loci of insertion were 1 Crispr Array of 216
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         <div class="body-title">Body</div>
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         <div class="body-title">Cas Proteins </div>
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        <div class="body-subtitle">Subtitle</div>
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         <div class="body-subtitle">Right Image</div>
 
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             Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam hendrerit, nunc vitae
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             The characterization of the production of proteins was mesure using the Biuret method to cuantify the total proteins under the induction with IPTG. Using controls of BL21 0 mM IPTG and BL21 transformed with Cas 1 and Cas 2 0 mM IPTG. We used different concetration of IPTG of 0 mM, 0.1 mM, 0.5 mM and 1 mM, and inducted for 6 hours taking samples every 30 min for 1 mM IPTG and every hour for the rest of the samples. Determining that the concentration of 0.1 mM has the highest concentration of total proteins.  
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             <img src="https://static.igem.org/mediawiki/2018/5/53/T--Tec-Monterrey--Imagen_Memo_Pensante.jpeg">
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             <img src="https://static.igem.org/mediawiki/2018/b/b8/T--Tec-Monterrey--Results_protein_production.jpeg">
 
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             <div class="leyenda">Figure 1: Normal Table with values</div>
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             <div class="leyenda">Figure 1: Production of Cas proteins under different concentration and time</div>
 
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         <div class="body-subtitle">Retron production </div>
 
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             Total RNA extraction was performed with IPTG induced E coli transformed with the msDNA construct. The samples were runned in a 8% polyacriliamide gel.We consider that those bands on the lines with RNAse A are the DNA section of our msDNA, which as ssDNA has a size of 117 bp, however due to its self complementarity. We consider that those bands with RNAse A are the DNA section of our msDNA, which as ssDNA has a size of 117 bp, however due to its self complementarity quality, it forms a hairpin with a size around 58 bp. Total RNA extraction was performed with IPTG induced E coli transformed with the msDNA construct. The samples were runned in a 8% polyacriliamide gel. We consider that those bands with RNAse A are the DNA section of our msDNA, which as ssDNA has a size of 117 bp, however due to its self complementarity quality, it forms a hairpin with a size around 58 bp.
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             <img src="https://static.igem.org/mediawiki/2018/5/53/T--Tec-Monterrey--Imagen_Memo_Pensante.jpeg">
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             <img src="https://static.igem.org/mediawiki/2018/b/b0/T--Tec-Monterrey--GELResults3.png">
 
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             <div class="leyenda">Figure 1: Normal Table with values</div>
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             <div class="leyenda">Figure 2: Retron Production</div>
 
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Revision as of 03:43, 18 October 2018

Overview
T
Cas Proteins
Right Image
The characterization of the production of proteins was mesure using the Biuret method to cuantify the total proteins under the induction with IPTG. Using controls of BL21 0 mM IPTG and BL21 transformed with Cas 1 and Cas 2 0 mM IPTG. We used different concetration of IPTG of 0 mM, 0.1 mM, 0.5 mM and 1 mM, and inducted for 6 hours taking samples every 30 min for 1 mM IPTG and every hour for the rest of the samples. Determining that the concentration of 0.1 mM has the highest concentration of total proteins.

Figure 1: Production of Cas proteins under different concentration and time

Retron production
Total RNA extraction was performed with IPTG induced E coli transformed with the msDNA construct. The samples were runned in a 8% polyacriliamide gel.We consider that those bands on the lines with RNAse A are the DNA section of our msDNA, which as ssDNA has a size of 117 bp, however due to its self complementarity. We consider that those bands with RNAse A are the DNA section of our msDNA, which as ssDNA has a size of 117 bp, however due to its self complementarity quality, it forms a hairpin with a size around 58 bp. Total RNA extraction was performed with IPTG induced E coli transformed with the msDNA construct. The samples were runned in a 8% polyacriliamide gel. We consider that those bands with RNAse A are the DNA section of our msDNA, which as ssDNA has a size of 117 bp, however due to its self complementarity quality, it forms a hairpin with a size around 58 bp.

Figure 2: Retron Production

Content Continues
erat. Phasellus et lorem mauris. Integer sit amet elit sed risus fermentum mollis eu eu mi. Praesent vestibulum ligula id purus convallis euismod. Vestibulum dignissim ante id tortor dapibus feugiat non eget ligula. Sed sit amet nisi ac sapien vehicula lobortis. Suspendisse quis libero at turpis venenatis bibendum sit amet at purus. Aliquam interdum puru non mollis molestie. Mauris quis felis vitae neque ornare commodo at id sapien. Vestibulum mattis id libero at suscipit.
Figure 1: Normal Table with values
Content Continues
erat. Phasellus et lorem mauris. Integer sit amet elit sed risus fermentum mollis eu eu mi. Praesent vestibulum ligula id purus convallis euismod. Vestibulum dignissim ante id tortor dapibus feugiat non eget ligula. Sed sit amet nisi ac sapien vehicula lobortis. Suspendisse quis libero at turpis venenatis bibendum sit amet at purus. Aliquam interdum puru non mollis molestie. Mauris quis felis vitae neque ornare commodo at id sapien. Vestibulum mattis id libero at suscipit.
Figure 1: Normal Table with values
Conclusion
Erat. Phasellus et lorem mauris. Integer sit amet elit sed risus fermentum mollis eu eu mi. Praesent vestibulum ligula id purus convallis euismod. Vestibulum dignissim ante id tortor dapibus feugiat non eget ligula. Sed sit amet nisi ac sapien vehicula lobortis. Suspendisse quis libero at turpis venenatis bibendum sit amet at purus. Aliquam interdum puru non mollis molestie. Mauris quis felis vitae neque ornare commodo at id sapien. Vestibulum mattis id libero at suscipit.
References
[1] Autor. (Year). Paper title. Magazine, volume(magazine number), pages. DOI or WEBPAGE
[1] Johnes G.M., Bails J. (2009). The Paper of Biology. McBookity Papers, 78(3), 215-200. DOI: 919kjaij19san.

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