Difference between revisions of "Team:Vilnius-Lithuania/Demonstrate"
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| − | <h1> | + | <h1>Proof</h1> |
| − | <p> | + | <p>The proof of SynDrop’s concept consists of several critical steps:</p> |
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| − | + | <li>We have specified the parameters for cell-sized liposome (5-30 µm) synthesis by using our COMSOL model.</li> | |
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| − | + | <p>We have concluded that modifications to microfluidic channels affect our system the most (5 µm change in width increased liposome diameter by almost 6 µm), while viscosity and flow rate regulations proved to be an efficient way to fine-tune liposome size in a range of few micrometers (Fig. 1). Moreover, critical value of flow rate ratio was found (Q = 0.7), at which liposomes stopped forming. | |
| − | + | </p> | |
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| − | + | <img src="https://static.igem.org/mediawiki/2018/e/ed/T--Vilnius-Lithuania--dv_fig1a_Model.gif" style="max-width:100%" /> | |
| − | + | <p><strong>Fig. 1 </strong> Visual representation of liposome formation based on baseline experimental parametric values. Phase variables A, B and C here have values of 1, 2 and 3 respectively.</p> | |
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Revision as of 21:16, 4 November 2018
Proof of Concept
The Composite Proof
We proved that our SynDrop system worked as intended by successfully implementing several critical wet lab and dry lab experiments. First, we have created a model to determine microfluidics variables for optimal liposome synthesis. Second, we synthesized stable biocompatible liposomes and demonstrated an internal transcription and translation of functional proteins. Third, we demonstrated that membrane proteins can successfully integrate into our liposomes. Finally, we constructed working fusion proteins that were able to display a designated tag on the outer membrane.
Proof
The proof of SynDrop’s concept consists of several critical steps:
- We have specified the parameters for cell-sized liposome (5-30 µm) synthesis by using our COMSOL model.
We have concluded that modifications to microfluidic channels affect our system the most (5 µm change in width increased liposome diameter by almost 6 µm), while viscosity and flow rate regulations proved to be an efficient way to fine-tune liposome size in a range of few micrometers (Fig. 1). Moreover, critical value of flow rate ratio was found (Q = 0.7), at which liposomes stopped forming.
Fig. 1 Visual representation of liposome formation based on baseline experimental parametric values. Phase variables A, B and C here have values of 1, 2 and 3 respectively.
invert
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