Difference between revisions of "Team:UCL/Parts"

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{{UCL/BootstrapCSS}}
 
{{UCL/BootstrapCSS}}
 
{{UCL/Style}}
 
{{UCL/Style}}
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<html lang="en">
 
<html lang="en">
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     <div class="dropdown-content_igem"id="dropdownmodelling_igem">
 
     <div class="dropdown-content_igem"id="dropdownmodelling_igem">
 
         <a href="https://2018.igem.org/Team:UCL/Model">Modelling</a>
 
         <a href="https://2018.igem.org/Team:UCL/Model">Modelling</a>
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        <a href="https://2018.igem.org/Team:UCL/Model#Intein">Intein</a>
 
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        <a href="https://2018.igem.org/Team:UCL/Model#Intein_Polymerisation">Intein Polymerisation</a>
 
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        <a href="https://2018.igem.org/Team:UCL/Model#LAMMPS">LAMMPS</a>
 
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        <a href="https://2018.igem.org/Team:UCL/Model#Scale_Up">Scale Up</a>
 
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </button>
 
     </button>
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     <div class="dropdown-content_igem"id"dropdownhumanpractices_igem">
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     <div class="dropdown-content_igem"id="dropdownhumanpractices_igem">
 
         <a href="https://2018.igem.org/Team:UCL/Human_Practices">Human Practices</a>
 
         <a href="https://2018.igem.org/Team:UCL/Human_Practices">Human Practices</a>
 
         <a href="https://2018.igem.org/Team:UCL/Public_Engagement">Public Engagement</a>
 
         <a href="https://2018.igem.org/Team:UCL/Public_Engagement">Public Engagement</a>
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     <div class="container container_igem">
 
     <div class="container container_igem">
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        <div id="sidenav" class="sidenav wow fadeInUp" data-wow-duration="2s" data-wow-delay="0.1s">
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              <a href="#Overview">Overview</a>
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              <a href="#BB2">BioBrick 2.0</a>
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              <a href="#Intein_Passenger">Intein Passenger</a>
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              <a href="#RFP">Intein Monomer 1 (RFP)</a>
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              <a href="#GFP">Intein Monomer 2 (GFP)</a>
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              <a href="#Spindroin">Spindroin</a>
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              <a href="#Functionalisation">Functionalisation</a>
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              <a href="#Basic_Parts">Basic Parts</a>
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              <a href="#Composite_Parts">Composite Parts</a>
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          </div>
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 +
        <section id="Overview">
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            <div class="card card_igem wow fadeInUp" data-wow-duration="2s" data-wow-delay="0.1s">
 +
                <div class="card-title text-center">
 +
                    <h4>Overview</h4>
 +
                </div>
 +
                <div class="card-body text-justify">
 +
                    <div class="container">
 +
                        <div class="row">
 +
 +
                                <p class="card-text card-text_igem2 text-justify">
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                                    Our BioBricks were created to be used together to enable the polymerisation and functionalisation of biomaterials. All our composite parts contain our BioBrick 2.0 standardisation, which combines canonical BioBrick prefix and suffix with Golden Gate compatible BsaI sites. This standardisation allows for more efficient and flexible cloning.
 +
                                  </p>
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                                  <p class="card-text card-text_igem2 text-justify"></p>
 +
                                    <p class="card-text card-text_igem2 text-justify">
 +
                                    To facilitate polymerisation and functionalisation, we created reporter devices flanked or capped by orthogonal inteins. These BioBricks contain SapI sites before and after their reporter sequence, to create plug and play systems where any material protein can be polymerised and any functionalising protein can be incorporated into the final biomaterial.
 +
                                  </p>
 +
                                  <p class="card-text card-text_igem2 text-justify"></p>
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                                    <p class="card-text card-text_igem2 text-justify">
 +
                                    Ultimately, the idea of utilising spider silk to create water filtration biomaterials influenced the design and creation of our BioBricks.
 +
                                    </p>
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                              </div>
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                            </div>
 +
                        </div>
 +
                    </div>
 +
 +
        </section>
 +
        <section id="BB2">
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            <div class="card card_igem wow fadeInUp" data-wow-duration="2s" data-wow-delay="0.1s">
 +
                <div class="card-title text-center">
 +
                    <h4>BioBrick 2.0</h4>
 +
                </div>
 +
                <div class="card-body text-justify">
 +
                    <div class="container">
 +
                        <div class="col-sm-10 mmmm">
 +
                            <img class="cards_logos" src="https://static.igem.org/mediawiki/2018/c/cd/T--UCL--FB.png" />
 +
                        </div>
 +
 +
                        <div class="row">
 +
                            <div class="col-sm-6">
 +
                                <p class="card-text card-text_igem text-justify">
 +
                                    This BioBrick-compatible standard with improved flexibility enables the integration of conventional cloning methods into iGEM’s workflow. Once inserted into a backbone, BioBrick2.0 allows cloning through Golden Gate assembly and Gibson assembly. At the same time, our construct has a LacZ reporter which can be used to screen plates for successful colonies. BioBrick2.0 is a new standard which can facilitate the cloning process for future iGEM teams.
 +
                                </p>
 +
                            </div>
 +
                         
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
            </div>
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        </section>
 
          
 
          
 +
 
<center>
 
<center>
 
     <section id="Basic_Parts">
 
     <section id="Basic_Parts">
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                             </tr>
 
                             </tr>
 
                             <tr>
 
                             <tr>
−
                                 <td class="tableleft"><a class="sshh" href="http://parts.igem.org/Part:BBa_K2842699">BBa_K2842699</a></td>
+
                                 <td class="tableleft"><a class="sshh" href="http://parts.igem.org/Part:BBa_K2842669">BBa_K2842669</a></td>
 
                                 <td class="tableleft">mScarlet reporter with AceL-TerL-C intein on the N terminus (intein passenger)</td>
 
                                 <td class="tableleft">mScarlet reporter with AceL-TerL-C intein on the N terminus (intein passenger)</td>
 
                                 <td class="tableright">1256</td>
 
                                 <td class="tableright">1256</td>
 
                             </tr>
 
                             </tr>
 
                             <tr>
 
                             <tr>
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                                 <td class="tableleft"><a class="sshh" href="http://parts.igem.org/Part:BBa_K2842705">BBa_K2842705</a></td>
+
                                 <td class="tableleft"><a class="sshh" href="http://parts.igem.org/Part:BBa_K2842680">BBa_K2842680</a></td>
 
                                 <td class="tableleft">Intein Monomer 1: RFP reporter flanked with orthogonal inteins (RFP intein)</td>
 
                                 <td class="tableleft">Intein Monomer 1: RFP reporter flanked with orthogonal inteins (RFP intein)</td>
 
                                 <td class="tableright">1197</td>
 
                                 <td class="tableright">1197</td>

Revision as of 14:43, 9 November 2018


UCL SETA - Parts

Parts

Overview

Our BioBricks were created to be used together to enable the polymerisation and functionalisation of biomaterials. All our composite parts contain our BioBrick 2.0 standardisation, which combines canonical BioBrick prefix and suffix with Golden Gate compatible BsaI sites. This standardisation allows for more efficient and flexible cloning.

To facilitate polymerisation and functionalisation, we created reporter devices flanked or capped by orthogonal inteins. These BioBricks contain SapI sites before and after their reporter sequence, to create plug and play systems where any material protein can be polymerised and any functionalising protein can be incorporated into the final biomaterial.

Ultimately, the idea of utilising spider silk to create water filtration biomaterials influenced the design and creation of our BioBricks.

BioBrick 2.0

This BioBrick-compatible standard with improved flexibility enables the integration of conventional cloning methods into iGEM’s workflow. Once inserted into a backbone, BioBrick2.0 allows cloning through Golden Gate assembly and Gibson assembly. At the same time, our construct has a LacZ reporter which can be used to screen plates for successful colonies. BioBrick2.0 is a new standard which can facilitate the cloning process for future iGEM teams.

BioBrick Description Length (bp)
BBa_K2842667 Flexible Cloning Region F 33
BBa_K2842668 Flexible Cloning Region R 33
BBa_K2842670 Lac Operator 17
BBa_K2842671 Ribosome binding site 24
BBa_K2842672 AceL-TerL-C intein 312
BBa_K2842673 Efficient Cleavage Site 9
BBa_K2842675 SapI Cassette F 15
BBa_K2842676 SapI Cassette R 15
BBa_K2842678 Spidroin N-terminus 402
BBa_K2842679 Efficient Cleavage site 9
BBa_K2842681 mRFP1 675
BBa_K2842682 Npu-C intein 108
BBa_K2842683 AceL-TerL-N intein 75
BBa_K2842684 Ribosome Binding Site 22
BBa_K2842685 Spacer 20
BBa_K2842691 Npu-N intein 312
BBa_K2842692 AceL-TerL-C intein 312
BBa_K2842693 Efficient Cloning Site for Npu-C intein 9
BBa_K2842694 Efficient Cloning Site for AceL-TerL-C intein 9
BBa_K2842695 SapI cassete GFP F 15
BBa_K2842696 SapI cassete GFP R 15
BBa_K2842697 Spacer 9
BBa_K2842698 RBS for GFP 27
BBa_K2842699 GFP codon optimised 714
BBa_K2842704 Ribosome binding site 18
BBa_K2842705 Npu-C intein 104
BBa_K2842706 Spidroin C-terminus 363
BBa_K2842707 Spacer 14
BBa_K2842708 SGS linker 9
BBa_K2842709 NikR C-terminal domain (E. coli) 255
BBa_K2842711 Estrogen receptor protein (M421F) 744

BBa_K2842666 Golden Gate compatible system with blue-white screening (BioBrick 2.0) 679
BBa_K2842669 mScarlet reporter with AceL-TerL-C intein on the N terminus (intein passenger) 1256
BBa_K2842680 Intein Monomer 1: RFP reporter flanked with orthogonal inteins (RFP intein) 1197
BBa_K2842690 Intein Monomer 2: GFP reporter flanked with orthogonal inteins (GFP intein) 1568
BBa_K2842700 N-Terminus with AceL-TerL-N intein (minispidroin NT) 597
BBa_K2842701 C-Terminus with Npu-C intein minispidroin CT) 721
BBa_K2842710 NikR with AceL-TerL-C intein 803
BBa_K2842720 Estrogen receptor protein (M421F) with TerL-C intein 1304
Our Location
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Contact Us
ucl.igem@ucl.ac.uk
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