Difference between revisions of "Team:IIT-Madras/Improvements"

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<li>Using thermodynamic data and correlate it with the sequence of the promoter region. The experimental and thermodynamic data of the sequences obtained can be used to correlate it with the expression levels of the protein. Come up with a model to understand the correlation between the sequence of promoter and expression rates.</li> <br>
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<li>Correlating thermodynamic data with the sequence of the promoter region: The experimental and thermodynamic data of the sequences obtained can be used to correlate it with the expression levels of the protein. A model can be built to understand the correlation between the promoter sequences and expression rates.</li> <br>
−
<li>Troubleshooting or try to understand the reason of extremely high expression rate of P8S. </li><br>
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<li>Troubleshooting, or trying to understand the reason for the extremely high expression rate of P8S. </li><br>
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<li>Characterize remaining biobrick parts.</li><br>
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<li>Characterizing the remaining BioBrick parts.</li><br>
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<li>Test promoter library in other chassis such as E. coli and other gram-negative microorganisms</li><br>
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<li>Testing the promoter library in other chassis such as <i>E. coli</i> and other gram-negative microorganisms.</li><br>
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<li>Use Codon usage table available for other organisms of industrial interest using CUTE.</li><br>
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<li>Generating and using codon-usage tables for other organisms of industrial interest using CUTE.</li><br>
 
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Revision as of 23:07, 7 December 2018

iGEM Collaborations Page

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Team: IIT-Madras/Improvements

Improvements

Further studies that we wish to do:

  • Correlating thermodynamic data with the sequence of the promoter region: The experimental and thermodynamic data of the sequences obtained can be used to correlate it with the expression levels of the protein. A model can be built to understand the correlation between the promoter sequences and expression rates.

  • Troubleshooting, or trying to understand the reason for the extremely high expression rate of P8S.

  • Characterizing the remaining BioBrick parts.

  • Testing the promoter library in other chassis such as E. coli and other gram-negative microorganisms.

  • Generating and using codon-usage tables for other organisms of industrial interest using CUTE.