Line 92: | Line 92: | ||
</div> | </div> | ||
<p>Our first day in the laboratory</p></div> | <p>Our first day in the laboratory</p></div> | ||
− | <div class="collapsible-body"><span> Goal: Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics.</span></div> | + | <div class="collapsible-body"><span> Goal: Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics. Preparation of LA- medium |
+ | LA- medium (1,5%) were made after the following recipe, and autoclaved for 20min at 121°C. | ||
+ | |||
+ | <ul> | ||
+ | <li> 1L Distilled water </li> | ||
+ | |||
+ | <li>5g Yeast extract </li> | ||
+ | |||
+ | <li>10g Tryptone </li> | ||
+ | |||
+ | <li>5g NaCl</li> | ||
+ | |||
+ | <li>15g Agar </li></span></div> | ||
</li> | </li> | ||
<li> | <li> |
Revision as of 12:08, 9 July 2018
Notebook
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Week25
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Jun18
Test
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Jun19
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Jun20
Our first day in the laboratory
Goal: Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics. Preparation of LA- medium LA- medium (1,5%) were made after the following recipe, and autoclaved for 20min at 121°C.- 1L Distilled water
- 5g Yeast extract
- 10g Tryptone
- 5g NaCl
- 15g Agar
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Jun21
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Jun22
Isolation and verification of pgRNA and pdCas9
Test
Week26Note title
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Jul2
InterLabs Study
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