Difference between revisions of "Team:NTNU Trondheim/Notebook"

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             <p>Inoculation of E.coli from agar plates to LB-medium</p></div>
 
             <p>Inoculation of E.coli from agar plates to LB-medium</p></div>
 
             <div class="collapsible-body"><span>
 
             <div class="collapsible-body"><span>
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<b>Goal:</b> Inoculate a colony from each agar plate which were prepared from the previous day, in LB- medium with antibiotics. <br>
+
<b>Goal:</b> Inoculate a colony from each agar plate which were prepared from the previous day, in LB- medium with antibiotics. <br> <br>
−
<b>Procedure:</b>
+
<b>Procedure</b> <br>
−
<br>
+
 
One colony of pgRNA- and pdCas9- bacteria were inoculated in LB-media (25mL) with AMP (25μL) and CM (25μL), respectively. The cell cultures were incubated at 37℃ in a shaking incubator at 204rpm. <br>
 
One colony of pgRNA- and pdCas9- bacteria were inoculated in LB-media (25mL) with AMP (25μL) and CM (25μL), respectively. The cell cultures were incubated at 37℃ in a shaking incubator at 204rpm. <br>
 
</span></div>
 
</span></div>

Revision as of 12:51, 9 July 2018

Notebook


  • Week
    25

    Note title

    • Jun
      18

      Test

      Test.
    • Jun
      19

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      20

      Our first day in the laboratory!

      Goal: Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics.
      Preparation of LA- medium
      LA- medium (1.5%) were made after the following recipe, and autoclaved for 20min at 121°C.
      • 1L Distilled water
      • 5g Yeast extract
      • 10g Tryptone
      • 5g NaCl
      • 15g Agar

      Agar plates with antibiotics
      Two different agar plates were made – one type contains ampicillin (AMP) while the other type contains chloramphenicol (CM), and were prepared after the following recipe:
      LA- medium with AMP:
      • 0.5L LA-medium
      • 0.5mL AMP (50mg/mL)

      LA-medium with CM:
      • 0.5L LA-medium
      • 0.5mL CM (35mg/mL)
      The LA-media with antibiotic were poured on petri dishes.
    • Jun
      21

      Inoculation of E.coli from agar plates to LB-medium

      Goal: Inoculate a colony from each agar plate which were prepared from the previous day, in LB- medium with antibiotics.

      Procedure
      One colony of pgRNA- and pdCas9- bacteria were inoculated in LB-media (25mL) with AMP (25μL) and CM (25μL), respectively. The cell cultures were incubated at 37℃ in a shaking incubator at 204rpm.
    • Jun
      22

      Isolation and verification of pgRNA and pdCas9

      Test
  • Week
    26

    Note title

    • Jun
      25

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      26

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      27

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      28

      Note title

      Lorem ipsum dolor sit amet.
    • Jun
      29

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    27

    Note title

    • Jul
      1

      Note title

    • Jul
      2

      InterLabs Study

      Calibration 1 and 2 for the InterLabs Study.
    • Jul
      3

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      4

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      5

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    28

    Note title

    • Jul
      9

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      10

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      11

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      12

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      13

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    29

    Note title

    • Jul
      16

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      17

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      18

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      19

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      20

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    30

    Note title

    • Jul
      23

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      24

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      25

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      26

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      27

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    31

    Note title

    • Jul
      30

      Note title

      Lorem ipsum dolor sit amet.
    • Jul
      31

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      1

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      2

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      3

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    32

    Note title

    • Aug
      6

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      7

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      8

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      9

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      10

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    33

    Note title

    • Aug
      13

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      14

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      15

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      16

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      17

      Note title

      Lorem ipsum dolor sit amet.
  • Week
    34

    Note title

    • Aug
      20

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      21

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      22

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      23

      Note title

      Lorem ipsum dolor sit amet.
    • Aug
      24

      Note title

      Lorem ipsum dolor sit amet.