Difference between revisions of "Team:CCU Taiwan/InterLab"

Background

All of the 2018 iGEM teams are invited and encouraged to participate in the Fourth International InterLaboratory Measurement Study in synthetic biology.” Our team took part in this study which aimed to be one of the labs to repeat measurements in different condition. In our case, we measured fluorescence using plate reader.

Interlab Project

OD 600 Reference point - LUDOX Protocol
Particle Standard Curve - Microsphere Protocol
Fluorescence standard curve - Fluorescein Protocol
Cell measurement protocol

Experiment

Calibration 1: OD 600 Reference point - LUDOX Protocol

Materials：

❏ 1ml LUDOX CL-X (provided in kit)
❏ ddH 2 0 (provided by team)
❏ 96 well plate, black with clear flat bottom preferred (provided by team)

Method：

❏ Add 100 μl LUDOX into wells A1, B1, C1, D1
❏ Add 100 μl of dd H 2 O into wells A2, B2, C2, D2
❏ Measure absorbance at 600 nm of all samples in the measurement mode you plan to use for cell measurements
❏ Record the data in the table below or in your notebook
❏ Import data into Excel sheet provided ( OD600 reference point tab )

Data Result：

Calibration 2: Particle Standard Curve - Microsphere Protocol

Materials：

❏ 300 μL Silica beads - Microsphere suspension (provided in kit, 4.7 x 10^8 microspheres)
❏ ddH 2 0 (provided by team)
❏ 96 well plate, black with clear flat bottom preferred (provided by team)

Method：

Prepare the Microsphere Stock Solution:
❏ Obtain the tube labeled “Silica Beads” from the Interlab test kit and vortex vigorously for 30 seconds.
NOTE: Microspheres should NOT be stored at -20 degrees C or below , as freezing affects the properties of the microspheres. If you believe your microspheres may have been frozen, please contact the iGEM Measurement Committee for a replacement (measurement at igem dot org).
❏ Immediately pipet 96 μL microspheres into a 1.5 mL eppendorf tube
❏ Add 904 μL of ddH 2 O to the microspheres
❏ Vortex well. This is your Microsphere Stock Solution

Data Result：