Difference between revisions of "Team:SDSZ China"

Line 6: Line 6:
  
  
 +
</body>
  
−
 
−
<div class="column full_size" >
 
 
<h1> Welcome to iGEM SDSZ_China! </h1>
 
<h1> Welcome to iGEM SDSZ_China! </h1>
−
<table width="200" border="1">
+
</br>
−
  <tbody>
+
<t>Chitin is an abundant natural macromolecular substances in the exoskeleton of arthropods. It could be converted by deacetylation to chitosan, which is -1, 4- polymer of 2-glucosamine. Chitosan is significantly soluble and bioactive, being widely used in medicine, cosmetics, food industry, water treatment and chemical industry. Treated with concentrated alkali, the current technology of chitosan production is deficient, unstable, squandering of energy, and, especially, contaminative.
−
    <tr>
+
−
      <td>&nbsp;</td>
+
−
    </tr>
+
−
    <tr>
+
−
      <td>&nbsp;</td>
+
−
    </tr>Chitin is an abundant natural macromolecular substances in the exoskeleton of arthropods. It could be converted by deacetylation to chitosan, which is -1, 4- polymer of 2-glucosamine. Chitosan is significantly soluble and bioactive, being widely used in medicine, cosmetics, food industry, water treatment and chemical industry. Treated with concentrated alkali, the current technology of chitosan production is deficient, unstable, squandering of energy, and, especially, contaminative.
+
 
According to a currently promising technology of deacetylation using chitin-active enzymes, we found out that Chitin Deacetylase (CDA) could hydrolyze the acetamino group on chitin, directly yielding chitin, and the only industrial-available source of chitin is the especially high-crystallized chitin from shell of crustacea like shrimp and crab. In order to applicate enzymolysis method to industry, separation, identification and production of crystal-chitin-active-enzymes is crucial, both promoting industrialization and giving instruction to scientific research.
 
According to a currently promising technology of deacetylation using chitin-active enzymes, we found out that Chitin Deacetylase (CDA) could hydrolyze the acetamino group on chitin, directly yielding chitin, and the only industrial-available source of chitin is the especially high-crystallized chitin from shell of crustacea like shrimp and crab. In order to applicate enzymolysis method to industry, separation, identification and production of crystal-chitin-active-enzymes is crucial, both promoting industrialization and giving instruction to scientific research.
 
In our research, we chose several CDA and chitinase sequences, synthesized them, complemented them with respective domains, and cultivated them in plasmid pET-28a. After inserting plasmids into competent cells and searching for optimal induction condition for expression, we would finally find out maximum viability and put the research to model for factory production.
 
In our research, we chose several CDA and chitinase sequences, synthesized them, complemented them with respective domains, and cultivated them in plasmid pET-28a. After inserting plasmids into competent cells and searching for optimal induction condition for expression, we would finally find out maximum viability and put the research to model for factory production.
−
 
+
</t>
−
  </tbody>
+
 
−
</table>
+
</body>
−
</div>
+
−
<div class="clear extra_space"></div>
+
−
<div class="line_divider"></div>
+
−
<div class="clear extra_space"></div>
+
−
<div class="clear extra_space"></div>
+
−
<div class="column third_size" > </div>
+
−
 
+
−
 
+
−
 
+
−
<div class="column third_size" > </div>
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
<div class="clear extra_space"></div>
+
−
<div class="line_divider"></div>
+
−
<div class="column third_size"> </div>
+
−
 
+
−
 
+
−
 
+
−
 
+
 
</html>
 
</html>

Revision as of 11:42, 21 July 2018


HOME
TEAM
Team Members
Collaborations
PROJECT
Description
Design
Experiments
Notebook
InterLab
Model
Results
Demonstrate
Improve
Attributions
PARTS
Parts Overview
Basic Parts
Composite Parts
Part Collection
SAFETY
HUMAN PRACTICES
Human Practices
Education & Engagement
AWARDS
Applied Design
Entrepreneurship
Hardware
Measurement
Model
Plant
Software
JUDGING FORM ⇗

Welcome to iGEM SDSZ_China!


Chitin is an abundant natural macromolecular substances in the exoskeleton of arthropods. It could be converted by deacetylation to chitosan, which is -1, 4- polymer of 2-glucosamine. Chitosan is significantly soluble and bioactive, being widely used in medicine, cosmetics, food industry, water treatment and chemical industry. Treated with concentrated alkali, the current technology of chitosan production is deficient, unstable, squandering of energy, and, especially, contaminative. According to a currently promising technology of deacetylation using chitin-active enzymes, we found out that Chitin Deacetylase (CDA) could hydrolyze the acetamino group on chitin, directly yielding chitin, and the only industrial-available source of chitin is the especially high-crystallized chitin from shell of crustacea like shrimp and crab. In order to applicate enzymolysis method to industry, separation, identification and production of crystal-chitin-active-enzymes is crucial, both promoting industrialization and giving instruction to scientific research. In our research, we chose several CDA and chitinase sequences, synthesized them, complemented them with respective domains, and cultivated them in plasmid pET-28a. After inserting plasmids into competent cells and searching for optimal induction condition for expression, we would finally find out maximum viability and put the research to model for factory production.