Difference between revisions of "Team:Rotterdam HR/Notebook"

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<h1 class="title">Stock up</h1>
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<h1 class="title">Architecture design [TC]</h1>
<h3 class="date">7/11/2018</h3>
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<h3 class="date">18-6-2018</h3>
<label class="back" for="switch-11564107">&times;</label>
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<label class="back" for="switch-67137454">&times;</label>
 
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<div class="attendees">Elise Grootscholten | Loraine Nelson | Paul Reusink</div>
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<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
<div class="description">To have some basic biobricks ready, we stocked them by doing a plasmid isolation and freeze the DNA. </div>
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<div class="description">The first draft of the architecture design for the temperature controller was made.</div>
 
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<div class="number"><b>01</b></div>
 
<div class="number"><b>01</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Stock up</h3>
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<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Architecture design [TC]</h3>
 
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<label for="switch-11564107" class="more-info">Show more</label>
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<label for="switch-67137454" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/11/2018</h2>
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<h2 class="date" style="color: white;">18-6-2018</h2>
 
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<h1 class="title">Basic parts</h1>
+
<h1 class="title">Architecture design [TC]</h1>
<h3 class="date">7/11/2018</h3>
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<h3 class="date">24-6-2018</h3>
<label class="back" for="switch-88607341">&times;</label>
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<label class="back" for="switch-81354866">&times;</label>
 
</div>
 
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<div class="attendees">Elise Grootscholten | Loraine Nelson | Paul Reusink</div>
+
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
<div class="description">To start on a basic construct we did a digestion and ligation with the parts we isolated earlier. To prevent original constructs we did a defosphorylation after the digestion. We controlled the digestion by doing a gelelktroforeses. </div>
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<div class="description">Today a few changes have been made to the architecture design of the temperature controller. The choice was made to use a premade power supply as opposed to a self-made one, because this was considering the time left to finish this device the best choice. A few alterations were made to make the architecture design better readable.</div>
 
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<div class="number"><b>01</b></div>
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<div class="number"><b>02</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Basic parts</h3>
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<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Architecture design [TC]</h3>
 
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<label for="switch-88607341" class="more-info">Show more</label>
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<label for="switch-81354866" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/11/2018</h2>
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<h2 class="date" style="color: white;">24-6-2018</h2>
 
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+
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</svg>
 
</svg>
<h1 class="title">Basic parts</h1>
+
<h1 class="title">First prototype [TC]</h1>
<h3 class="date">7/12/2018</h3>
+
<h3 class="date">25-6-2018</h3>
<label class="back" for="switch-29560433">&times;</label>
+
<label class="back" for="switch-40301340">&times;</label>
 
</div>
 
</div>
<div class="attendees">Tom van Dijk | Randall de Waard</div>
+
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
<div class="description">We continued with the ligated DNA by transformating it to Neb10Beta. This was plated onto agarplates and incubated for 1 day. </div>
+
<div class="description">A 3d drawing was made for the casing that will hold the temperature controller. The first prototype was printed, but was determent to be inadequate due to the lack of enough space for the cooler block.</div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
<div class="number"><b>02</b></div>
+
<div class="number"><b>01</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Basic parts</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">First prototype [TC]</h3>
 
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+
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</svg>
<label for="switch-29560433" class="more-info">Show more</label>
+
<label for="switch-40301340" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/12/2018</h2>
+
<h2 class="date" style="color: white;">25-6-2018</h2>
 
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+
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<h1 class="title">Basic parts</h1>
+
<h1 class="title">Second prototype [TC]</h1>
<h3 class="date">7/13/2018</h3>
+
<h3 class="date">26-6-2018</h3>
<label class="back" for="switch-59850373">&times;</label>
+
<label class="back" for="switch-79768518">&times;</label>
 
</div>
 
</div>
<div class="attendees">Elise Grootscholten | Randall de Waard</div>
+
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
<div class="description">The results of 7/12/2018 were collected, in action of this we decided to plate some ligated and transferred DNA from 7/12/2018 again. This we did because there wasn't a clear grow of colonies on the plates.</div>
+
<div class="description">In the drawing space was added to fit the cooler block for the temperature controller. Slits were added to the drawing for the nuts to slide into. The altered drawing was printed. The newly printed parts, cooler block, fan and Peltier element were assembled. Everything fit together.</div>
 
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<div class="cardform">
 
<div class="cardform">
<div class="number"><b>03</b></div>
+
<div class="number"><b>02</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Basic parts</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Second prototype [TC]</h3>
 
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<svg style="width:80px;height:80px" viewBox="0 0 24 24">
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+
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<label for="switch-59850373" class="more-info">Show more</label>
+
<label for="switch-79768518" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/13/2018</h2>
+
<h2 class="date" style="color: white;">26-6-2018</h2>
 
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+
<path fill="currentColor" d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path>
 
</svg>
 
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<h1 class="title">Paper Bacteria</h1>
+
<h1 class="title">Testrun [TC]</h1>
<h3 class="date">7/13/2018</h3>
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<h3 class="date">27-6-2018</h3>
<label class="back" for="switch-75799824">&times;</label>
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<label class="back" for="switch-69002062">&times;</label>
 
</div>
 
</div>
<div class="attendees">Elise Grootscholten | Randall de Waard</div>
+
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
<div class="description">We tried to let bacteria grow on paper, therefore we used our own buisnesscards. We let the buisnesscards absorb some LB-medium and put them inside petridishes. </div>
+
<div class="description">A short test run was done with the newly assembled cooler. The test concluded that the temperature controller could cool a water droplet to the point of freezing and heat it back up to its boiling point.</div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
<div class="number"><b>01</b></div>
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<div class="number"><b>03</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Paper Bacteria</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Testrun [TC]</h3>
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
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+
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</svg>
 
</svg>
<label for="switch-75799824" class="more-info">Show more</label>
+
<label for="switch-69002062" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/13/2018</h2>
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<h2 class="date" style="color: white;">27-6-2018</h2>
 
</div>
 
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+
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</svg>
 
</svg>
<h1 class="title">Basic parts</h1>
+
<h1 class="title">Draft decision</h1>
<h3 class="date">7/16/2018</h3>
+
<h3 class="date">2-7-2018</h3>
<label class="back" for="switch-75432063">&times;</label>
+
<label class="back" for="switch-64497069">&times;</label>
 
</div>
 
</div>
<div class="attendees">Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Randall de Waard</div>
+
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
<div class="description">To know if the ligation was succesfull we will controll the DNA, therefor we ented the bacteria's with DNA in LB-medium to grow overnight. We also plated the used bacteria's onto new plates to use for further experiments. </div>
+
<div class="description">The choice to use a computer PSU as a power supply was made. The choice was made on the grounds, that computer PSU have a standard 12, 5 and 3.3V output. This is beneficial because these are the voltages necessary for the selected components.</div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
<div class="number"><b>04</b></div>
+
<div class="number"><b>01</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Basic parts</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Draft decision</h3>
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
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+
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</svg>
<label for="switch-75432063" class="more-info">Show more</label>
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<label for="switch-64497069" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/16/2018</h2>
+
<h2 class="date" style="color: white;">2-7-2018</h2>
 
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+
<path fill="currentColor" d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path>
 
</svg>
 
</svg>
<h1 class="title">Paper Bacteria </h1>
+
<h1 class="title">Architecture design [TC]</h1>
<h3 class="date">7/16/2018</h3>
+
<h3 class="date">5-7-2018</h3>
<label class="back" for="switch-51076740">&times;</label>
+
<label class="back" for="switch-50188109">&times;</label>
 
</div>
 
</div>
<div class="attendees">Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Randall de Waard</div>
+
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
<div class="description">We did a blue white screening on our buisnesscards to see if bacteria had grew on them. We transferred the buisnesscards to new petrydishes and added ITPG and X-gal to the petrydishes. </div>
+
<div class="description">Changes were made to the architecture design of the temperature controller. Different voltage regulators were selected to fit the newly chosen power supply. To better readability of the architecture design a legend was added.</div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
<div class="number"><b>02</b></div>
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<div class="number"><b>03</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Paper Bacteria </h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Architecture design [TC]</h3>
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
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+
<path fill="currentColor" d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path>
 
</svg>
 
</svg>
<label for="switch-51076740" class="more-info">Show more</label>
+
<label for="switch-50188109" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/16/2018</h2>
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<h2 class="date" style="color: white;">5-7-2018</h2>
 
</div>
 
</div>
  
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<style>
 
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<div class="card wetlab-card" id="card-82007518">
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<div class="card hardware-card" id="card-10047631">
  
 
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+
<path fill="currentColor" d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path>
 
</svg>
 
</svg>
<h1 class="title">Basic parts</h1>
+
<h1 class="title">Electrical circuit design [TC]</h1>
<h3 class="date">7/17/2018</h3>
+
<h3 class="date">8-7-2018</h3>
<label class="back" for="switch-82007518">&times;</label>
+
<label class="back" for="switch-10047631">&times;</label>
 
</div>
 
</div>
<div class="attendees">Rens Boeser | Dustin van der Meulen | Loraine Nelson  | Suzanne Romeijn | Randall de Waard</div>
+
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
<div class="description">The resulst of 7/16/2018 showed that the DNA didnt ligate as planned.  To be able to digest enough DNA we did a plasmid isolation again. We digested this DNA and froze it for further use. </div>
+
<div class="description">Custom footprints were made for multiple components and added to the parts library in the PCB design software.</div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
<div class="number"><b>05</b></div>
+
<div class="number"><b>01</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Basic parts</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Electrical circuit design [TC]</h3>
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
+
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<label for="switch-82007518" class="more-info">Show more</label>
+
<label for="switch-10047631" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/17/2018</h2>
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<h2 class="date" style="color: white;">8-7-2018</h2>
 
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<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
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<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
+
<path fill="currentColor" d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path>
 
</svg>
 
</svg>
<h1 class="title">stocking</h1>
+
<h1 class="title">Electrical circuit design [TC]</h1>
<h3 class="date">7/18/2018</h3>
+
<h3 class="date">9-7-2018</h3>
<label class="back" for="switch-92131419">&times;</label>
+
<label class="back" for="switch-53339477">&times;</label>
 
</div>
 
</div>
<div class="attendees">Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Mike Vrieswijk | Randall de Waard</div>
+
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
<div class="description">We did another round of plasmid isolation for stocking. </div>
+
<div class="description">The provisional electronic circuit design for the temperature controller was made.</div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
 
<div class="number"><b>02</b></div>
 
<div class="number"><b>02</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">stocking</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Electrical circuit design [TC]</h3>
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
+
<path fill="currentColor" d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path>
 
</svg>
 
</svg>
<label for="switch-92131419" class="more-info">Show more</label>
+
<label for="switch-53339477" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/18/2018</h2>
+
<h2 class="date" style="color: white;">9-7-2018</h2>
 
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<style>
 
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#switch-66844997:checked ~ #card-66844997 {
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#switch-66844997:checked ~ #card-66844997 .entryform {
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#switch-66844997:checked ~ #card-66844997 .cardform,
+
#switch-79064080:checked ~ #card-79064080 .cardform,
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+
#switch-79064080:checked ~ #card-79064080::before,
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+
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</style>
 
</style>
  
<input type="checkbox" id="switch-66844997" class="switch"></input>
+
<input type="checkbox" id="switch-79064080" class="switch"></input>
<div class="card wetlab-card" id="card-66844997">
+
<div class="card wetlab-card" id="card-79064080">
  
 
<div class="entryform">
 
<div class="entryform">
Line 694: Line 694:
 
<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
</svg>
 
</svg>
<h1 class="title">Basic parts</h1>
+
<h1 class="title">Stock up</h1>
<h3 class="date">7/19/2018</h3>
+
<h3 class="date">11-7-2018</h3>
<label class="back" for="switch-66844997">&times;</label>
+
<label class="back" for="switch-79064080">&times;</label>
 
</div>
 
</div>
<div class="attendees">Dustin van der Meulen | Mei Ju Goemans| Randall de Waard </div>
+
<div class="attendees">Elise Grootscholten | Loraine Nelson | Paul Reusink</div>
<div class="description">We digested all DNA we have in stock to test for our basic construct. We tested the DNA by doing a gelelktroforeses. </div>
+
<div class="description">To have some basic biobricks ready, we stocked them by doing a plasmid isolation and freeze the DNA. </div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
<div class="number"><b>07</b></div>
+
<div class="number"><b>01</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Basic parts</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Stock up</h3>
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
</svg>
 
</svg>
<label for="switch-66844997" class="more-info">Show more</label>
+
<label for="switch-79064080" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/19/2018</h2>
+
<h2 class="date" style="color: white;">11-7-2018</h2>
 
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<style>
 
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#switch-41603227:checked ~ #card-41603227 {
+
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width: 100%;
 
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height: auto;
 
height: auto;
 
}
 
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#switch-41603227:checked ~ #card-41603227 .entryform {
+
#switch-22525914:checked ~ #card-22525914 .entryform {
 
display: block;
 
display: block;
 
}
 
}
#switch-41603227:checked ~ #card-41603227 .cardform,
+
#switch-22525914:checked ~ #card-22525914 .cardform,
#switch-41603227:checked ~ #card-41603227::before,
+
#switch-22525914:checked ~ #card-22525914::before,
#switch-41603227:checked ~ #card-41603227::after {
+
#switch-22525914:checked ~ #card-22525914::after {
 
display: none;
 
display: none;
 
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</style>
 
</style>
  
<input type="checkbox" id="switch-41603227" class="switch"></input>
+
<input type="checkbox" id="switch-22525914" class="switch"></input>
<div class="card wetlab-card" id="card-41603227">
+
<div class="card wetlab-card" id="card-22525914">
  
 
<div class="entryform">
 
<div class="entryform">
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<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
</svg>
 
</svg>
<h1 class="title">ATP sensor</h1>
+
<h1 class="title">Basic parts</h1>
<h3 class="date">7/24/2018</h3>
+
<h3 class="date">11-7-2018</h3>
<label class="back" for="switch-41603227">&times;</label>
+
<label class="back" for="switch-22525914">&times;</label>
 
</div>
 
</div>
<div class="attendees">Rens Boeser | Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Randall de Waard</div>
+
<div class="attendees">Elise Grootscholten | Loraine Nelson | Paul Reusink</div>
<div class="description">New biobricks, used to make an ATP sensor, were transformed into NEB10Beta. The culture was than plated onto agar and incubated for 1 day. </div>
+
<div class="description">To start on a basic construct we did a digestion and ligation with the parts we isolated earlier. To prevent original constructs we did a defosphorylation after the digestion. We controlled the digestion by doing a gelelktroforeses. </div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
 
<div class="number"><b>01</b></div>
 
<div class="number"><b>01</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">ATP sensor</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Basic parts</h3>
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
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</svg>
 
</svg>
<label for="switch-41603227" class="more-info">Show more</label>
+
<label for="switch-22525914" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/24/2018</h2>
+
<h2 class="date" style="color: white;">11-7-2018</h2>
 
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<input type="checkbox" id="switch-61174767" class="switch"></input>
+
<input type="checkbox" id="switch-80749137" class="switch"></input>
<div class="card wetlab-card" id="card-61174767">
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<div class="card hardware-card" id="card-80749137">
  
 
<div class="entryform">
 
<div class="entryform">
 
<div class="titlebar">
 
<div class="titlebar">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
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+
<path fill="currentColor" d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path>
 
</svg>
 
</svg>
<h1 class="title">Gas output</h1>
+
<h1 class="title">Electrical circuit design [TC]</h1>
<h3 class="date">7/25/2018</h3>
+
<h3 class="date">11-7-2018</h3>
<label class="back" for="switch-61174767">&times;</label>
+
<label class="back" for="switch-80749137">&times;</label>
 
</div>
 
</div>
<div class="attendees">Rens Boeser | Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Randall de Waard</div>
+
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
<div class="description">New biobricks, which produces gasses, were transformed into NEB10Beta. The culture was than plated onto agar and incubated for 1 day. </div>
+
<div class="description">The electronic circuit design for the temperature controller was finished. Measuring point were added to the electronic circuit design.</div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
<div class="number"><b>01</b></div>
+
<div class="number"><b>03</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Gas output</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Electrical circuit design [TC]</h3>
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
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+
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</svg>
 
</svg>
<label for="switch-61174767" class="more-info">Show more</label>
+
<label for="switch-80749137" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/25/2018</h2>
+
<h2 class="date" style="color: white;">11-7-2018</h2>
 
</div>
 
</div>
  
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+
<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
</svg>
 
</svg>
<h1 class="title">Architecture design [TC]</h1>
+
<h1 class="title">Basic parts</h1>
<h3 class="date">6/18/2018</h3>
+
<h3 class="date">12-7-2018</h3>
<label class="back" for="switch-23933109">&times;</label>
+
<label class="back" for="switch-50519131">&times;</label>
 
</div>
 
</div>
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
+
<div class="attendees">Tom van Dijk | Randall de Waard</div>
<div class="description">The first draft of the architecture design for the temperature controller was made.</div>
+
<div class="description">We continued with the ligated DNA by transformating it to Neb10Beta. This was plated onto agarplates and incubated for 1 day. </div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
<div class="number"><b>01</b></div>
+
<div class="number"><b>02</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Architecture design [TC]</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Basic parts</h3>
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
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+
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<label for="switch-23933109" class="more-info">Show more</label>
+
<label for="switch-50519131" class="more-info">Show more</label>
<h2 class="date" style="color: white;">6/18/2018</h2>
+
<h2 class="date" style="color: white;">12-7-2018</h2>
 
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<h1 class="title">Architecture design [TC]</h1>
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<h1 class="title">Board layout [TC]</h1>
<h3 class="date">6/24/2018</h3>
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<h3 class="date">12-7-2018</h3>
<label class="back" for="switch-19749984">&times;</label>
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<label class="back" for="switch-93399282">&times;</label>
 
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<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
 
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
<div class="description">Today a few changes have been made to the architecture design of the temperature controller. The choice was made to use a premade power supply as opposed to a self-made one, because this was considering the time left to finish this device the best choice. A few alterations were made to make the architecture design better readable.</div>
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<div class="description">Started working on the board layout for the PCB of the temperature controller.</div>
 
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<div class="number"><b>02</b></div>
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<div class="number"><b>01</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Architecture design [TC]</h3>
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<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Board layout [TC]</h3>
 
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<label for="switch-19749984" class="more-info">Show more</label>
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<label for="switch-93399282" class="more-info">Show more</label>
<h2 class="date" style="color: white;">6/24/2018</h2>
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<h2 class="date" style="color: white;">12-7-2018</h2>
 
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<h1 class="title">First prototype [TC]</h1>
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<h1 class="title">Basic parts</h1>
<h3 class="date">6/25/2018</h3>
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<h3 class="date">13-7-2018</h3>
<label class="back" for="switch-92076834">&times;</label>
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<label class="back" for="switch-64162985">&times;</label>
 
</div>
 
</div>
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
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<div class="attendees">Elise Grootscholten | Randall de Waard</div>
<div class="description">A 3d drawing was made for the casing that will hold the temperature controller. The first prototype was printed, but was determent to be inadequate due to the lack of enough space for the cooler block.</div>
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<div class="description">The results of 7/12/2018 were collected, in action of this we decided to plate some ligated and transferred DNA from 7/12/2018 again. This we did because there wasn't a clear grow of colonies on the plates.</div>
 
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<div class="number"><b>01</b></div>
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<div class="number"><b>03</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">First prototype [TC]</h3>
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<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Basic parts</h3>
 
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<label for="switch-92076834" class="more-info">Show more</label>
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<label for="switch-64162985" class="more-info">Show more</label>
<h2 class="date" style="color: white;">6/25/2018</h2>
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<h2 class="date" style="color: white;">13-7-2018</h2>
 
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</svg>
<h1 class="title">Second prototype [TC]</h1>
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<h1 class="title">Paper Bacteria</h1>
<h3 class="date">6/26/2018</h3>
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<h3 class="date">13-7-2018</h3>
<label class="back" for="switch-85208181">&times;</label>
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<label class="back" for="switch-44425296">&times;</label>
 
</div>
 
</div>
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
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<div class="attendees">Elise Grootscholten | Randall de Waard</div>
<div class="description">In the drawing space was added to fit the cooler block for the temperature controller. Slits were added to the drawing for the nuts to slide into. The altered drawing was printed. The newly printed parts, cooler block, fan and Peltier element were assembled. Everything fit together.</div>
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<div class="description">We tried to let bacteria grow on paper, therefore we used our own buisnesscards. We let the buisnesscards absorb some LB-medium and put them inside petridishes. </div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
<div class="number"><b>02</b></div>
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<div class="number"><b>01</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Second prototype [TC]</h3>
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<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Paper Bacteria</h3>
 
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<label for="switch-85208181" class="more-info">Show more</label>
+
<label for="switch-44425296" class="more-info">Show more</label>
<h2 class="date" style="color: white;">6/26/2018</h2>
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<h2 class="date" style="color: white;">13-7-2018</h2>
 
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<h1 class="title">Testrun [TC]</h1>
+
<h1 class="title">Board layout [TC]</h1>
<h3 class="date">6/27/2018</h3>
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<h3 class="date">13-7-2018</h3>
<label class="back" for="switch-34359305">&times;</label>
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<label class="back" for="switch-31449028">&times;</label>
 
</div>
 
</div>
 
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
 
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
<div class="description">A short test run was done with the newly assembled cooler. The test concluded that the temperature controller could cool a water droplet to the point of freezing and heat it back up to its boiling point.</div>
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<div class="description">Further work was done to the board layout for the temperature controller.</div>
 
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<div class="cardform">
<div class="number"><b>03</b></div>
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<div class="number"><b>02</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Testrun [TC]</h3>
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<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Board layout [TC]</h3>
 
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<label for="switch-34359305" class="more-info">Show more</label>
+
<label for="switch-31449028" class="more-info">Show more</label>
<h2 class="date" style="color: white;">6/27/2018</h2>
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<h2 class="date" style="color: white;">13-7-2018</h2>
 
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<h1 class="title">Draft decision</h1>
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<h1 class="title">Board layout [TC]</h1>
<h3 class="date">7/2/2018</h3>
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<h3 class="date">15-7-2018</h3>
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<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
 
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
<div class="description">The choice to use a computer PSU as a power supply was made. The choice was made on the grounds, that computer PSU have a standard 12, 5 and 3.3V output. This is beneficial because these are the voltages necessary for the selected components.</div>
+
<div class="description">Work has been done on the board layout for the temperature controller. The board layout is now finished.</div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
<div class="number"><b>01</b></div>
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<div class="number"><b>03</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Draft decision</h3>
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<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Board layout [TC]</h3>
 
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<label for="switch-97934435" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/2/2018</h2>
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<h2 class="date" style="color: white;">15-7-2018</h2>
 
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</svg>
<h1 class="title">Architecture design [TC]</h1>
+
<h1 class="title">Basic parts</h1>
<h3 class="date">7/5/2018</h3>
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<h3 class="date">16-7-2018</h3>
<label class="back" for="switch-74270954">&times;</label>
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<label class="back" for="switch-29942014">&times;</label>
 
</div>
 
</div>
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
+
<div class="attendees">Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Randall de Waard</div>
<div class="description">Changes were made to the architecture design of the temperature controller. Different voltage regulators were selected to fit the newly chosen power supply. To better readability of the architecture design a legend was added.</div>
+
<div class="description">To know if the ligation was succesfull we will controll the DNA, therefor we ented the bacteria's with DNA in LB-medium to grow overnight. We also plated the used bacteria's onto new plates to use for further experiments. </div>
 
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<div class="cardform">
<div class="number"><b>03</b></div>
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<div class="number"><b>04</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Architecture design [TC]</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Basic parts</h3>
 
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<h2 class="date" style="color: white;">7/5/2018</h2>
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<h2 class="date" style="color: white;">16-7-2018</h2>
 
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<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
</svg>
 
</svg>
<h1 class="title">Electrical circuit design [TC]</h1>
+
<h1 class="title">Paper Bacteria </h1>
<h3 class="date">7/8/2018</h3>
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<h3 class="date">16-7-2018</h3>
<label class="back" for="switch-55261906">&times;</label>
+
<label class="back" for="switch-30086432">&times;</label>
 
</div>
 
</div>
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
+
<div class="attendees">Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Randall de Waard</div>
<div class="description">Custom footprints were made for multiple components and added to the parts library in the PCB design software.</div>
+
<div class="description">We did a blue white screening on our buisnesscards to see if bacteria had grew on them. We transferred the buisnesscards to new petrydishes and added ITPG and X-gal to the petrydishes. </div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
<div class="number"><b>01</b></div>
+
<div class="number"><b>02</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Electrical circuit design [TC]</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Paper Bacteria </h3>
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
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+
<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
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<label for="switch-55261906" class="more-info">Show more</label>
+
<label for="switch-30086432" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/8/2018</h2>
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<h2 class="date" style="color: white;">16-7-2018</h2>
 
</div>
 
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Line 1,145: Line 1,145:
  
 
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<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
</svg>
 
</svg>
<h1 class="title">Electrical circuit design [TC]</h1>
+
<h1 class="title">Basic parts</h1>
<h3 class="date">7/9/2018</h3>
+
<h3 class="date">17-7-2018</h3>
<label class="back" for="switch-68513563">&times;</label>
+
<label class="back" for="switch-98769459">&times;</label>
 
</div>
 
</div>
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
+
<div class="attendees">Rens Boeser | Dustin van der Meulen | Loraine Nelson  | Suzanne Romeijn | Randall de Waard</div>
<div class="description">The provisional electronic circuit design for the temperature controller was made.</div>
+
<div class="description">The resulst of 7/16/2018 showed that the DNA didnt ligate as planned.  To be able to digest enough DNA we did a plasmid isolation again. We digested this DNA and froze it for further use. </div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
<div class="number"><b>02</b></div>
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<div class="number"><b>05</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Electrical circuit design [TC]</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Basic parts</h3>
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
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+
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<label for="switch-68513563" class="more-info">Show more</label>
+
<label for="switch-98769459" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/9/2018</h2>
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<h2 class="date" style="color: white;">17-7-2018</h2>
 
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Line 1,188: Line 1,188:
  
 
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+
<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
</svg>
 
</svg>
<h1 class="title">Electrical circuit design [TC]</h1>
+
<h1 class="title">stocking</h1>
<h3 class="date">7/11/2018</h3>
+
<h3 class="date">18-7-2018</h3>
<label class="back" for="switch-60942739">&times;</label>
+
<label class="back" for="switch-95424158">&times;</label>
 
</div>
 
</div>
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
+
<div class="attendees">Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Mike Vrieswijk | Randall de Waard</div>
<div class="description">The electronic circuit design for the temperature controller was finished. Measuring point were added to the electronic circuit design.</div>
+
<div class="description">We did another round of plasmid isolation for stocking. </div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
<div class="number"><b>03</b></div>
+
<div class="number"><b>02</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Electrical circuit design [TC]</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">stocking</h3>
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
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+
<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
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<label for="switch-60942739" class="more-info">Show more</label>
+
<label for="switch-95424158" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/11/2018</h2>
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<h2 class="date" style="color: white;">18-7-2018</h2>
 
</div>
 
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Line 1,231: Line 1,231:
  
 
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<div class="card wetlab-card" id="card-76578208">
  
 
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<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
</svg>
 
</svg>
<h1 class="title">Board layout [TC]</h1>
+
<h1 class="title">Basic parts</h1>
<h3 class="date">7/12/2018</h3>
+
<h3 class="date">19-7-2018</h3>
<label class="back" for="switch-70519699">&times;</label>
+
<label class="back" for="switch-76578208">&times;</label>
 
</div>
 
</div>
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
+
<div class="attendees">Dustin van der Meulen | Mei Ju Goemans| Randall de Waard </div>
<div class="description">Started working on the board layout for the PCB of the temperature controller.</div>
+
<div class="description">We digested all DNA we have in stock to test for our basic construct. We tested the DNA by doing a gelelktroforeses. </div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
<div class="number"><b>01</b></div>
+
<div class="number"><b>07</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Board layout [TC]</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Basic parts</h3>
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
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+
<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
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<label for="switch-70519699" class="more-info">Show more</label>
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<label for="switch-76578208" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/12/2018</h2>
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<h2 class="date" style="color: white;">19-7-2018</h2>
 
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+
<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
</svg>
 
</svg>
<h1 class="title">Board layout [TC]</h1>
+
<h1 class="title">ATP sensor</h1>
<h3 class="date">7/13/2018</h3>
+
<h3 class="date">24-7-2018</h3>
<label class="back" for="switch-30565351">&times;</label>
+
<label class="back" for="switch-74520903">&times;</label>
 
</div>
 
</div>
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
+
<div class="attendees">Rens Boeser | Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Randall de Waard</div>
<div class="description">Further work was done to the board layout for the temperature controller.</div>
+
<div class="description">New biobricks, used to make an ATP sensor, were transformed into NEB10Beta. The culture was than plated onto agar and incubated for 1 day. </div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
<div class="number"><b>02</b></div>
+
<div class="number"><b>01</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Board layout [TC]</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">ATP sensor</h3>
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
<path fill="currentColor" d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path>
+
<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
</svg>
 
</svg>
<label for="switch-30565351" class="more-info">Show more</label>
+
<label for="switch-74520903" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/13/2018</h2>
+
<h2 class="date" style="color: white;">24-7-2018</h2>
 
</div>
 
</div>
  
Line 1,317: Line 1,317:
  
 
<style>
 
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+
#switch-12556158:checked ~ #card-12556158 {
 
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#switch-46939876:checked ~ #card-46939876 .entryform {
+
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#switch-46939876:checked ~ #card-46939876 .cardform,
+
#switch-12556158:checked ~ #card-12556158 .cardform,
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+
#switch-12556158:checked ~ #card-12556158::before,
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+
#switch-12556158:checked ~ #card-12556158::after {
 
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<input type="checkbox" id="switch-46939876" class="switch"></input>
+
<input type="checkbox" id="switch-12556158" class="switch"></input>
<div class="card hardware-card" id="card-46939876">
+
<div class="card wetlab-card" id="card-12556158">
  
 
<div class="entryform">
 
<div class="entryform">
 
<div class="titlebar">
 
<div class="titlebar">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
<path fill="currentColor" d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path>
+
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</svg>
 
</svg>
<h1 class="title">Board layout [TC]</h1>
+
<h1 class="title">Gas output</h1>
<h3 class="date">7/15/2018</h3>
+
<h3 class="date">25-7-2018</h3>
<label class="back" for="switch-46939876">&times;</label>
+
<label class="back" for="switch-12556158">&times;</label>
 
</div>
 
</div>
<div class="attendees">Tom van Dijk | Mike Vrieswijk</div>
+
<div class="attendees">Rens Boeser | Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Randall de Waard</div>
<div class="description">Work has been done on the board layout for the temperature controller. The board layout is now finished.</div>
+
<div class="description">New biobricks, which produces gasses, were transformed into NEB10Beta. The culture was than plated onto agar and incubated for 1 day. </div>
 
</div>
 
</div>
  
 
<div class="cardform">
 
<div class="cardform">
<div class="number"><b>03</b></div>
+
<div class="number"><b>01</b></div>
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Board layout [TC]</h3>
+
<h3 class="title" style="color: white; padding: 10px 5px 0 5px;">Gas output</h3>
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
 
<svg style="width:80px;height:80px" viewBox="0 0 24 24">
<path fill="currentColor" d="M15.9,18.45C17.25,18.45 18.35,17.35 18.35,16C18.35,14.65 17.25,13.55 15.9,13.55C14.54,13.55 13.45,14.65 13.45,16C13.45,17.35 14.54,18.45 15.9,18.45M21.1,16.68L22.58,17.84C22.71,17.95 22.75,18.13 22.66,18.29L21.26,20.71C21.17,20.86 21,20.92 20.83,20.86L19.09,20.16C18.73,20.44 18.33,20.67 17.91,20.85L17.64,22.7C17.62,22.87 17.47,23 17.3,23H14.5C14.32,23 14.18,22.87 14.15,22.7L13.89,20.85C13.46,20.67 13.07,20.44 12.71,20.16L10.96,20.86C10.81,20.92 10.62,20.86 10.54,20.71L9.14,18.29C9.05,18.13 9.09,17.95 9.22,17.84L10.7,16.68L10.65,16L10.7,15.31L9.22,14.16C9.09,14.05 9.05,13.86 9.14,13.71L10.54,11.29C10.62,11.13 10.81,11.07 10.96,11.13L12.71,11.84C13.07,11.56 13.46,11.32 13.89,11.15L14.15,9.29C14.18,9.13 14.32,9 14.5,9H17.3C17.47,9 17.62,9.13 17.64,9.29L17.91,11.15C18.33,11.32 18.73,11.56 19.09,11.84L20.83,11.13C21,11.07 21.17,11.13 21.26,11.29L22.66,13.71C22.75,13.86 22.71,14.05 22.58,14.16L21.1,15.31L21.15,16L21.1,16.68M6.69,8.07C7.56,8.07 8.26,7.37 8.26,6.5C8.26,5.63 7.56,4.92 6.69,4.92A1.58,1.58 0 0,0 5.11,6.5C5.11,7.37 5.82,8.07 6.69,8.07M10.03,6.94L11,7.68C11.07,7.75 11.09,7.87 11.03,7.97L10.13,9.53C10.08,9.63 9.96,9.67 9.86,9.63L8.74,9.18L8,9.62L7.81,10.81C7.79,10.92 7.7,11 7.59,11H5.79C5.67,11 5.58,10.92 5.56,10.81L5.4,9.62L4.64,9.18L3.5,9.63C3.41,9.67 3.3,9.63 3.24,9.53L2.34,7.97C2.28,7.87 2.31,7.75 2.39,7.68L3.34,6.94L3.31,6.5L3.34,6.06L2.39,5.32C2.31,5.25 2.28,5.13 2.34,5.03L3.24,3.47C3.3,3.37 3.41,3.33 3.5,3.37L4.63,3.82L5.4,3.38L5.56,2.19C5.58,2.08 5.67,2 5.79,2H7.59C7.7,2 7.79,2.08 7.81,2.19L8,3.38L8.74,3.82L9.86,3.37C9.96,3.33 10.08,3.37 10.13,3.47L11.03,5.03C11.09,5.13 11.07,5.25 11,5.32L10.03,6.06L10.06,6.5L10.03,6.94Z"></path>
+
<path fill="currentColor" d="M9.46,6.28L11.05,9C8.47,9.26 6.5,11.41 6.5,14A5,5 0 0,0 11.5,19C13.55,19 15.31,17.77 16.08,16H13.5V14H21.5V16H19.25C18.84,17.57 17.97,18.96 16.79,20H19.5V22H3.5V20H6.21C4.55,18.53 3.5,16.39 3.5,14C3.5,10.37 5.96,7.2 9.46,6.28M12.74,2.07L13.5,3.37L14.36,2.87L17.86,8.93L14.39,10.93L10.89,4.87L11.76,4.37L11,3.07L12.74,2.07Z" />
 
</svg>
 
</svg>
<label for="switch-46939876" class="more-info">Show more</label>
+
<label for="switch-12556158" class="more-info">Show more</label>
<h2 class="date" style="color: white;">7/15/2018</h2>
+
<h2 class="date" style="color: white;">25-7-2018</h2>
 
</div>
 
</div>
  

Revision as of 09:27, 25 July 2018

Architecture design [TC]

18-6-2018

Tom van Dijk | Mike Vrieswijk
The first draft of the architecture design for the temperature controller was made.
01

Architecture design [TC]

18-6-2018

Architecture design [TC]

24-6-2018

Tom van Dijk | Mike Vrieswijk
Today a few changes have been made to the architecture design of the temperature controller. The choice was made to use a premade power supply as opposed to a self-made one, because this was considering the time left to finish this device the best choice. A few alterations were made to make the architecture design better readable.
02

Architecture design [TC]

24-6-2018

First prototype [TC]

25-6-2018

Tom van Dijk | Mike Vrieswijk
A 3d drawing was made for the casing that will hold the temperature controller. The first prototype was printed, but was determent to be inadequate due to the lack of enough space for the cooler block.
01

First prototype [TC]

25-6-2018

Second prototype [TC]

26-6-2018

Tom van Dijk | Mike Vrieswijk
In the drawing space was added to fit the cooler block for the temperature controller. Slits were added to the drawing for the nuts to slide into. The altered drawing was printed. The newly printed parts, cooler block, fan and Peltier element were assembled. Everything fit together.
02

Second prototype [TC]

26-6-2018

Testrun [TC]

27-6-2018

Tom van Dijk | Mike Vrieswijk
A short test run was done with the newly assembled cooler. The test concluded that the temperature controller could cool a water droplet to the point of freezing and heat it back up to its boiling point.
03

Testrun [TC]

27-6-2018

Draft decision

2-7-2018

Tom van Dijk | Mike Vrieswijk
The choice to use a computer PSU as a power supply was made. The choice was made on the grounds, that computer PSU have a standard 12, 5 and 3.3V output. This is beneficial because these are the voltages necessary for the selected components.
01

Draft decision

2-7-2018

Architecture design [TC]

5-7-2018

Tom van Dijk | Mike Vrieswijk
Changes were made to the architecture design of the temperature controller. Different voltage regulators were selected to fit the newly chosen power supply. To better readability of the architecture design a legend was added.
03

Architecture design [TC]

5-7-2018

Electrical circuit design [TC]

8-7-2018

Tom van Dijk | Mike Vrieswijk
Custom footprints were made for multiple components and added to the parts library in the PCB design software.
01

Electrical circuit design [TC]

8-7-2018

Electrical circuit design [TC]

9-7-2018

Tom van Dijk | Mike Vrieswijk
The provisional electronic circuit design for the temperature controller was made.
02

Electrical circuit design [TC]

9-7-2018

Stock up

11-7-2018

Elise Grootscholten | Loraine Nelson | Paul Reusink
To have some basic biobricks ready, we stocked them by doing a plasmid isolation and freeze the DNA.
01

Stock up

11-7-2018

Basic parts

11-7-2018

Elise Grootscholten | Loraine Nelson | Paul Reusink
To start on a basic construct we did a digestion and ligation with the parts we isolated earlier. To prevent original constructs we did a defosphorylation after the digestion. We controlled the digestion by doing a gelelktroforeses.
01

Basic parts

11-7-2018

Electrical circuit design [TC]

11-7-2018

Tom van Dijk | Mike Vrieswijk
The electronic circuit design for the temperature controller was finished. Measuring point were added to the electronic circuit design.
03

Electrical circuit design [TC]

11-7-2018

Basic parts

12-7-2018

Tom van Dijk | Randall de Waard
We continued with the ligated DNA by transformating it to Neb10Beta. This was plated onto agarplates and incubated for 1 day.
02

Basic parts

12-7-2018

Board layout [TC]

12-7-2018

Tom van Dijk | Mike Vrieswijk
Started working on the board layout for the PCB of the temperature controller.
01

Board layout [TC]

12-7-2018

Basic parts

13-7-2018

Elise Grootscholten | Randall de Waard
The results of 7/12/2018 were collected, in action of this we decided to plate some ligated and transferred DNA from 7/12/2018 again. This we did because there wasn't a clear grow of colonies on the plates.
03

Basic parts

13-7-2018

Paper Bacteria

13-7-2018

Elise Grootscholten | Randall de Waard
We tried to let bacteria grow on paper, therefore we used our own buisnesscards. We let the buisnesscards absorb some LB-medium and put them inside petridishes.
01

Paper Bacteria

13-7-2018

Board layout [TC]

13-7-2018

Tom van Dijk | Mike Vrieswijk
Further work was done to the board layout for the temperature controller.
02

Board layout [TC]

13-7-2018

Board layout [TC]

15-7-2018

Tom van Dijk | Mike Vrieswijk
Work has been done on the board layout for the temperature controller. The board layout is now finished.
03

Board layout [TC]

15-7-2018

Basic parts

16-7-2018

Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Randall de Waard
To know if the ligation was succesfull we will controll the DNA, therefor we ented the bacteria's with DNA in LB-medium to grow overnight. We also plated the used bacteria's onto new plates to use for further experiments.
04

Basic parts

16-7-2018

Paper Bacteria

16-7-2018

Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Randall de Waard
We did a blue white screening on our buisnesscards to see if bacteria had grew on them. We transferred the buisnesscards to new petrydishes and added ITPG and X-gal to the petrydishes.
02

Paper Bacteria

16-7-2018

Basic parts

17-7-2018

Rens Boeser | Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Randall de Waard
The resulst of 7/16/2018 showed that the DNA didnt ligate as planned. To be able to digest enough DNA we did a plasmid isolation again. We digested this DNA and froze it for further use.
05

Basic parts

17-7-2018

stocking

18-7-2018

Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Mike Vrieswijk | Randall de Waard
We did another round of plasmid isolation for stocking.
02

stocking

18-7-2018

Basic parts

19-7-2018

Dustin van der Meulen | Mei Ju Goemans| Randall de Waard
We digested all DNA we have in stock to test for our basic construct. We tested the DNA by doing a gelelktroforeses.
07

Basic parts

19-7-2018

ATP sensor

24-7-2018

Rens Boeser | Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Randall de Waard
New biobricks, used to make an ATP sensor, were transformed into NEB10Beta. The culture was than plated onto agar and incubated for 1 day.
01

ATP sensor

24-7-2018

Gas output

25-7-2018

Rens Boeser | Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Randall de Waard
New biobricks, which produces gasses, were transformed into NEB10Beta. The culture was than plated onto agar and incubated for 1 day.
01

Gas output

25-7-2018