Difference between revisions of "Team:BCU/Description"

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  <div class="nav">Nicotine degradation</div>
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    <div class="logo auto mb20"><a href="#"><img src="https://static.igem.org/mediawiki/2018/6/6a/T--BCU--bculogo.png" /></a></div>
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        <a href="https://2018.igem.org/Team:BCU#" class="on">HOME</a>
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        <a href="#">TEAM</a>
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        <a href="https://2018.igem.org/Team:BCU/Description">PROJECT</a>
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        <a href="#">PARTS</a>
 +
        <a href="#">SAFETY</a>
 +
        <a href="#">HUMAN PRACTICES</a>
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        <a href="#">AWARDS</a>
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Revision as of 01:57, 27 July 2018

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JUDGING FORM ⇗
Nicotine degradation

Description

1

Nicotine (1-methyl-2-[3-pyridyl-pyrrolidine], C10H14N2), a major alkaloid in tobacco plants, is a significant factor of evaluation for tobacco and cigarettes. Nicotine plays a critical role in smoking addiction and is well known to be harmful to human beings, because it easily crosses the blood-brain barrier and biological membranes. Meanwhile, with large quantities of tobacco products being produced and consumed, tobacco waste, which includes many toxic substances such as nicotine, aminobiphenyl, naphthylamine, and benzo(a)- pyrene, is entering the environment. Moreover, a number of toxic and harmful substances containing nicotine and other alkaloids are generated in tobacco processing and cannot be recycled, producing serious environmental problems.

2

In recent years, more nicotine-degrading bacteria have been reported, and the degradation pathways of nicotine have been elucidated. The degradation of nicotine through the pyrrolidine pathway by Pseudomonas has been extensively studied.

3

In our study， the first three gene NicA, pnao and sapd is crucial for nicotine degradation, so we will construct a pathway to express the three gene in top10.

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