Difference between revisions of "Team:TecMonterrey GDL/InterLab"

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<h3>Bronze Medal Criterion #4</h3>
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<p>This year (2018) the iGEM Team TecMonterrey_GDL decided to contribute into the Interlab study measurements. The goal of the study is summarized in achieving a way to reduce lab-to-lab variability in fluorescence measurements via normalizing to absolute cell count or colony forming units. The approaches taken were indicated by the iGEM measurement team:
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data aaaaaaaaaaaaaaaaaaaaaaaaafor an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
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For teams participating in the <a href="https://2018.igem.org/Measurement/InterLab">InterLab study</a>, all work must be shown on this page.
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1. Converting between absorbance of cells to absorbance of a known concentration of microsphere silica beads
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2. Counting colony-forming units (CFU) from the samples
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The team followed a series of protocols created to help standardize the measurement of GFP (green fluorescent protein), which is widely used as measurement marker. 
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Specifications:
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The equipment we used was Cytation 3 by Biotek. It can measure both absorbance and fluorescence. And the pathlength correction was disabled. The temperature of the measurements was done at room temperature.
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Calibration Protocol 1: OD600 Reference point  - LUDOX Protocol
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The first protocol’s goal was to obtain a conversion factor between absorbance measured and the OD600 measurement as it occurs in spectrophotometry measurements.
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This was made with the kit provided LUDOX CL-X (45% colloidal silica suspension) provided by iGEM and sterile mili Q water, reactants were added accordingly to the protocol and the obtained results are shown in the next table (Table 1):
 
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Revision as of 02:18, 28 July 2018

InterLab

This year (2018) the iGEM Team TecMonterrey_GDL decided to contribute into the Interlab study measurements. The goal of the study is summarized in achieving a way to reduce lab-to-lab variability in fluorescence measurements via normalizing to absolute cell count or colony forming units. The approaches taken were indicated by the iGEM measurement team: 1. Converting between absorbance of cells to absorbance of a known concentration of microsphere silica beads 2. Counting colony-forming units (CFU) from the samples The team followed a series of protocols created to help standardize the measurement of GFP (green fluorescent protein), which is widely used as measurement marker. Specifications: The equipment we used was Cytation 3 by Biotek. It can measure both absorbance and fluorescence. And the pathlength correction was disabled. The temperature of the measurements was done at room temperature. Calibration Protocol 1: OD600 Reference point - LUDOX Protocol The first protocol’s goal was to obtain a conversion factor between absorbance measured and the OD600 measurement as it occurs in spectrophotometry measurements. This was made with the kit provided LUDOX CL-X (45% colloidal silica suspension) provided by iGEM and sterile mili Q water, reactants were added accordingly to the protocol and the obtained results are shown in the next table (Table 1):