Difference between revisions of "Team:iTesla-SoundBio/Project/Interlab"

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  <a href="https://2018.igem.org/Team:iTesla-SoundBio/team">Team</a>
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  <a href="https://2018.igem.org/Team:iTesla-SoundBio/description">Description</a>
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  <a href="https://2018.igem.org/Team:iTesla-SoundBio/design">Design</a>
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  <a href="https://2018.igem.org/Team:iTesla-SoundBio/Experiment">Experiment</a>
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  <a href="https://2018.igem.org/Team:iTesla-SoundBio/Project/Interlab">InterLab</a>
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  <a href="https://2018.igem.org/Team:iTesla-SoundBio/Project/results">Results</a>
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  <a href="https://2018.igem.org/Team:iTesla-SoundBio/Project/Parts">Parts</a>
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  <a href="https://2018.igem.org/Team:iTesla-SoundBio/Project/Safety">Safety</a>
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  <a href="https://2018.igem.org/Team:iTesla-SoundBio/OldMainPage.html">Old Main Page</a>
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<h2>Team: iTesla-Soundbio</h2>
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<p>DID YOU KNOW? Every year, hundreds of thousands of horseshoe crabs are caught each year and drained of up to to 30% of their blood. Why? To collect LAL, a chemical that is crucial in the detection of endotoxins in everything from drugs to medical equipment.</p>
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  <h2>Project Outline</h2>
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<div class="row">
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<div class="column" style="background-color:#aaa;"><div class= "container">
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<a href="https://2018.igem.org/Team:iTesla-SoundBio/labnotebook">
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<img src="https://static.igem.org/mediawiki/2018/c/c1/T--iTesla-Soundbio--writing1.jpg" alt= "labnotebook", width= 100%, height=65%>
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<div class="centered">LabNotebook</div></div></div>
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<div class="column" style="background-color:#bbb;"><div class= "container1">
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<a href="https://2018.igem.org/Team:iTesla-SoundBio/a_crab_story">
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<img src="https://static.igem.org/mediawiki/2018/e/e1/T--iTesla-Soundbio--horseshoecrab1.jpg" alt="WE TAKE BLOOD FROM HORSESHOE CRABS", width= 100%, height=65%>
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<div class="centered">Fate of Horseshoe Crab</div></div></div>
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<div class="column" style="background-color:#ccc;"><div class= "container">
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<a href="https://2018.igem.org/Team:iTesla-SoundBio/gallary">
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<div class="centered">Human Practices</div></div></div>
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</div>
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<h1 style="color:#58D68D;">iTesla-SoundBio InterLab Study</h1>
 
<h1 style="color:#58D68D;">iTesla-SoundBio InterLab Study</h1>

Revision as of 02:38, 31 July 2018

Team Description Design Experiment InterLab Results Parts Safety Old Main Page

Team: iTesla-Soundbio

DID YOU KNOW? Every year, hundreds of thousands of horseshoe crabs are caught each year and drained of up to to 30% of their blood. Why? To collect LAL, a chemical that is crucial in the detection of endotoxins in everything from drugs to medical equipment.

Project Outline

labnotebook
LabNotebook
WE TAKE BLOOD FROM HORSESHOE CRABS
Fate of Horseshoe Crab
Human Practices

iTesla-SoundBio InterLab Study

Summary: For the 2018 InterLab study, high school students at iTesla-SoundBio prepared various colonies and dilutions to test for trends absorbance, fluorescence, and concentration. Massive thanks to the Washington iGEM InterLab team for guiding us through the protocol and lending us their plate readers. Below, is the timeline that we followed in completing the InterLab protocol.

ludoxprotocol

Part One- LUDOX Calibration Protocol

96 Well Plate Table

1 2 3 4 5 6 7 8 9 10 11 12
A LUDOX ddH2O
B LUDOX ddH2O
C LUDOX ddH2O
D LUDOX ddH2O
E
F
G
H

OD600 Reference Point Data

Here are the results for the Absorbance 600 of the LUDOX protcol

ludoxprotocol

Part Two- Microsphere Protocol

microspherecurve microsphere log curve

Part Three- Fluorescein Protocol

fluoresciencurve microsphere log curve

Bronze Medal Criterion #4

Standard Tracks: Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.

For teams participating in the InterLab study , all work must be shown on this page.