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| − | {{Ecuador}}
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| − | <html>
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| − | <style>
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| − | @import url('https://fonts.googleapis.com/css?family=Questrial');
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| − | *{
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| − | margin: 0px;
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| − | font-family: 'Questrial', sans-serif;
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| − | }
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| − | @keyframes fade-in {
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| − | from {
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| − | opacity: 0;
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| − | }
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| | | | |
| − | to {
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| − | opacity: 1;
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| − | }
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| − | }
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| − | @keyframes slide-in {
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| − | from {
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| − | margin-left: -100%;
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| − | }
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| − |
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| − | to {
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| − | margin-left: 0%;
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| − | }
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| − | }
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| − | .igem_2018_team_menu.displaying_menu {
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| − | display: none;
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| − | }
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| − | .ecuador_menu {
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| − | width: 100%;
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| − | height: 100px;
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| − | position: fixed;
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| − | left: 0px;
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| − | top: 15px;
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| − | background-color: #FFFFFF;
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| − | animation-name: fade-in;
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| − | animation-duration: 2s;
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| − | -webkit-box-shadow: 0px 3px 11px 0px rgba(0,0,0,0.75);
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| − | -moz-box-shadow: 0px 3px 11px 0px rgba(0,0,0,0.75);
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| − | box-shadow: 0px 3px 11px 0px rgba(0,0,0,0.75);
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| − | display: flex;
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| − | z-index: 5;
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| − | }
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| − |
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| − | .ecuador_home {
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| − | width: 16%;
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| − | height: 100%;
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| − | display: flex;
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| − | justify-content: center;
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| − | background-color: #154360;
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| − | position: absolute;
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| − | align-items: center;
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| − | margin: auto auto;
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| − | left: 1.5%;
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| − | color: #FFF;
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| − | transition: 0.5s;
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| − | -webkit-box-shadow: inset -60px -33px 154px -24px rgba(33,47,60,1);
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| − | -moz-box-shadow: inset -60px -33px 154px -24px rgba(33,47,60,1);
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| − | box-shadow: inset -60px -33px 154px -24px rgba(33,47,60,1);
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| − | }
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| − |
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| − | .ecuador_home:hover {
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| − | cursor: pointer;
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| − | font-size: 15px;
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| − | background-color: #154360;
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| − | box-shadow: none;
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| − | }
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| − |
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| − | .menu_options_container {
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| − | width: 79%;
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| − | height: 50px;
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| − | position: absolute;
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| − | left: 19%;
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| − | top: 25px;
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| − | display: flex;
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| − | justify-content: center;
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| − | }
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| − |
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| − | .menu_option {
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| − | width: 16.66%;
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| − | height: 100%;
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| − | position: relative;
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| − | justify-content: center;
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| − | align-items: center;
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| − | display: flex;
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| − | color: #797D7F;
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| − | transition: 0.2s;
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| − | text-align: center;
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| − | }
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| − |
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| − | .ecuador_href_text {
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| − | width: 16.66% !important;
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| − | height: 100% !important;
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| − | position: relative !important;
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| − | justify-content: center !important;
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| − | align-items: center !important;
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| − | display: flex !important;
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| − | color: #797D7F !important;
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| − | transition: 0.2s !important;
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| − | text-align: center !important;
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| − | text-decoration: none !important;
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| − | }
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| − |
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| − | .ecuador_href_sub_text {
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| − | width: 100% !important;
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| − | height: 40px !important;
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| − | position: relative !important;
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| − | text-align: left !important;
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| − | text-indent: 20px !important;
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| − | display: flex !important;
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| − | background-color: #000 !important;
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| − | margin: auto auto !important;
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| − | align-items: center !important;
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| − | transition: 0.2s !important;
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| − | color: #D5DBDB !important;
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| − | text-decoration: none !important;
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| − | }
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| − |
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| − | .menu_option:hover {
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| − | margin-top: 3px;
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| − | cursor: pointer;
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| − | }
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| − |
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| − | .menu_option:hover div.menu_sub_options_container{
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| − | display: block;
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| − | }
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| − |
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| − | .menu_sub_options_container {
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| − | width: 90%;
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| − | height: auto;
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| − | position: absolute;
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| − | top: 50px;
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| − | background-color: #FFF;
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| − | border-top: solid 1px;
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| − | display: none;
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| − | }
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| − |
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| − | .sub_menu_option {
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| − | width: 100%;
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| − | height: 40px;
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| − | position: relative;
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| − | text-align: left;
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| − | text-indent: 20px;
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| − | display: flex;
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| − | background-color: #000;
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| − | margin: auto auto;
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| − | align-items: center;
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| − | transition: 0.2s;
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| − | color: #D5DBDB;
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| − | }
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| − |
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| − | .sub_menu_option:hover {
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| − | text-indent: 25px;
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| − | }
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| − | .ecuador_footer {
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| − | width: 100%;
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| − | height: 250px;
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| − | position: absolute;
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| − | left: 0px;
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| − | top: 810px;
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| − | background-color: #212F3C;
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| − | }
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| − |
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| − | .ecuador_footer_igem_logo {
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| − | width: 180px;
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| − | height: 180px;
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| − | position: absolute;
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| − | right: 80px;
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| − | top: 35px;
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| − | background-image: url('https://static.igem.org/mediawiki/2018/5/55/T--Ecuador--iGEM--Logo--Black.png');
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| − | background-size: 100%;
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| − | background-repeat: no-repeat;
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| − | background-position: center;
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| − | }
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| − |
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| − | .ecuador_footer_information_layout {
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| − | width: 75%;
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| − | height: 80%;
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| − | position: absolute;
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| − | left: 60px;
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| − | top: 15%;
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| − | display: flex;
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| − | }
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| − |
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| − | .ecuador_footer_information_container {
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| − | width: 33.33%;
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| − | height: 100%;
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| − | position: relative;
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| − | }
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| − |
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| − | .ecuador_footer_information_title_container {
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| − | width: 100%;
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| − | height: 25px;
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| − | position: relative;
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| − | top: 0px;
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| − | text-align: center;
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| − | font-size: 20px;
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| − | font-weight: bold;
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| − | color: #B2BABB;
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| − | }
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| − |
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| − | .ecuador_follow_us_icons_container {
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| − | width: 50%;
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| − | height: 60px;
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| − | position: relative;
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| − | top: 40px;
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| − | margin: auto;
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| − | display: flex;
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| − | }
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| − |
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| − | .ecuador_follow_us_icon {
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| − | width: 60px;
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| − | height: 60px;
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| − | position: relative;
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| − | margin: auto;
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| − | }
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| − |
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| − |
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| − | #facebook {
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| − | background-image: url(https://static.igem.org/mediawiki/2018/4/42/T--Ecuador--Facebook.png);
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| − | background-repeat: no-repeat;
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| − | background-position: center;
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| − | background-size: 80%;
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| − | transition: 0.4s;
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| − | border-radius: 5px;
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| − | }
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| − |
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| − | #facebook:hover {
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| − | background-image: url(https://static.igem.org/mediawiki/2018/4/40/T--Ecuador--Facebook--White.png);
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| − | cursor: pointer;
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| − | }
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| − |
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| − | #instagram {
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| − | background-image: url(https://static.igem.org/mediawiki/2018/9/90/T--Ecuador--Instagram.png);
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| − | background-repeat: no-repeat;
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| − | background-position: center;
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| − | background-size: 80%;
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| − | transition: 0.4s;
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| − | border-radius: 5px;
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| − | }
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| − |
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| − | #instagram:hover {
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| − | background-image: url(https://static.igem.org/mediawiki/2018/4/49/T--Ecuador--Instagram--White.png);
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| − | cursor: pointer;
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| − | }
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| − |
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| − | .ecuador_abstract_container {
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| − | width: 100%;
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| − | height: 700px;
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| − | position: absolute;
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| − | left: 0px;
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| − | top: 110px;
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| − | background-color: #21618C;
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| − | }
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| − |
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| − | .ecuador_abstract_title_cotainer {
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| − | width: 100%;
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| − | height: 70px;
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| − | position: relative;
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| − | top: 30px;
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| − | margin: auto;
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| − | color: #FFF;
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| − | font-size: 32px;
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| − | display: flex;
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| − | justify-content: center;
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| − | align-items: center;
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| − | animation-name: slide-in;
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| − | animation-duration: 1.5s;
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| − | }
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| − |
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| − | .ecuador_abstract_text_container {
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| − | width: 100%;
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| − | height: 400px;
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| − | top: 70px;
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| − | position: relative;
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| − | }
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| − |
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| − | .ecuador_abstract_text {
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| − | width: 60%;
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| − | height: 100%;
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| − | position: relative;
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| − | margin: auto;
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| − | background-color: #21618C;
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| − | border-radius: 20px;
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| − | color: #FFF;
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| − | text-align: justify;
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| − | font-size: 20px;
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| − | animation-name: fade-in;
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| − | animation-duration: 4.5s;
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| − | }
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| − |
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| − | </style>
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| − |
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| − | <div class="ecuador_menu">
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| − | <a href="https://2018.igem.org/Team:Ecuador">
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| − | <div class="ecuador_home">
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| − | iGEM ECUADOR
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| − | </div>
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| − | </a>
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| − | <div class="menu_options_container">
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| − | <div id="project" class="menu_option">
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| − | <a class="ecuador_href_text" href="https://2018.igem.org/Team:Ecuador/Description">PROJECT</a>
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| − | <div class="menu_sub_options_container">
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| − | <div class="sub_menu_option">
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| − | MODELING
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| − | </div>
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| − | <div class="sub_menu_option">
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| − | DESIGN
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| − | </div>
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| − | <a class="ecuador_href_sub_text" href="https://2018.igem.org/Team:Ecuador/Background">
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| − | <div class="sub_menu_option">
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| − | BACKGROUND
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| − | </div>
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| − | </a>
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| − | </div>
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| − | </div>
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| − | <div id="people" class="menu_option">
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| − | PEOPLE
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| − | <div class="menu_sub_options_container">
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| − | <div class="sub_menu_option">
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| − | SPONSIRING
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| − | </div>
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| − | <div class="sub_menu_option">
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| − | ATTRIBUTIONS
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| − | </div>
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| − | <div class="sub_menu_option">
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| − | TEAM
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| − | </div>
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| − | </div>
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| − | </div>
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| − | <div id="notebook" class="menu_option">
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| − | NOTEBOOK
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| − | <div class="menu_sub_options_container">
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| − | <div class="sub_menu_option">
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| − | LAB BOOK
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| − | </div>
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| − | <div class="sub_menu_option">
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| − | PROTOCOLS
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| − | </div>
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| − | </div>
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| − | </div>
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| − | <div id="human_practice" class="menu_option">
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| − | HUMAN PRACTICE
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| − | <div class="menu_sub_options_container">
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| − | <div class="sub_menu_option">
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| − | COLLABORATIONS
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| − | </div>
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| − | </div>
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| − | </div>
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| − | <div id="parts" class="menu_option">
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| − | PARTS
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| − | <div class="menu_sub_options_container">
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| − | <div class="sub_menu_option">
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| − | LIST
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| − | </div>
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| − | <div class="sub_menu_option">
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| − | COMPOSITE
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| − | </div>
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| − | <div class="sub_menu_option">
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| − | BASIC
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| − | </div>
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| − | </div>
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| − | </div>
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| − | <div id="safety" class="menu_option">
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| − | SAFETY
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| − | </div>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="ecuador_abstract_container">
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| − | <div class="ecuador_abstract_title_cotainer">
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| − | PROJECT OVERVIEW
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| − | </div>
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| − | <div class="ecuador_abstract_text_container">
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| − | <div class="ecuador_abstract_text">
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| − | Biomaterials have the ability to interact with biological systems without being rejected. Through interdisciplinary research that involves cell biology, materials science, and bioengineering, biomaterials have been developed as biological substitutes to restore normal cellular function of organs and tissues. A new biomaterial will be developed based on the cross-linking of bacterial cellulose (BC) and elastin-like polypeptides (ELP), for biomedical applications. The cross-linking of both materials will be carried out with synthetic biology techniques using Escherichia coli as expression system. The crosslinking will be mediated by a carbohydrate binding domain protein module (CBD) specific for cellulose. CBDs are protein modules found in carbohydratases whose function is to bind carbohydrate to bring it closer to the catalytic site of the enzyme. For this process, three expression vectors will be designed. The first one will code for the enzymes necessary in the synthesis of bacterial cellulose, the second for the optimization of extracellular production and transport of bacterial cellulose and the third one will code for a fusion protein formed by the elastin-like polypeptide and the carbohydrate binding domain (CBD-ELP). A mathematical model for the interaction between cellulose and the CBD-ELP polypeptide will be determined based on the results from in-vitro experiments. The product generated by the modified bacteria will be characterized by biochemical methods and studied using structural elucidation methods, including spectrophotometry, nuclear magnetic resonance, mass spectrometry, and X-ray diffraction and its morphology will be elucidated using of atomic force microscopy. We expect that the new biomaterial combine the structural properties of bacterial cellulose with the functionalization capacity and thermo-sensitivity of elastin, for applications in food and tissue engineering, regenerative medicine, and selective release of drugs.
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| − | </div>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="ecuador_footer">
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| − | <div class="ecuador_footer_igem_logo">
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| − | </div>
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| − | <div class="ecuador_footer_information_layout">
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| − | <div class="ecuador_footer_information_container">
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| − | <div class="ecuador_footer_information_title_container">
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| − | SPONSORS
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| − | </div>
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| − | </div>
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| − | <div class="ecuador_footer_information_container">
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| − | <div class="ecuador_footer_information_title_container">
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| − | FOLLOW US
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| − | <div class="ecuador_follow_us_icons_container">
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| − | <div id="facebook" class="ecuador_follow_us_icon">
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| − |
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| − | </div>
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| − | <div id="instagram" class="ecuador_follow_us_icon">
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| − |
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| − | </div>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | <div class="ecuador_footer_information_container">
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| − | <div class="ecuador_footer_information_title_container">
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| − | CONTACT US
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| − | </div>
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| − | </div>
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| − | </div>
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| − | </div>
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| − |
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| − | </html>
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