Difference between revisions of "Team:Tec-Chihuahua/prueba"

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        <h3>José Pablo Rascón Pérez</h3>
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        <p>I’m 21 years old, currently in my 7th semester as a student of biotechnology engineering. Some of the things I like are: playing basketball, graphic design, science, playing  soccer, camping, fishing, exercising and being around the people that I love. This is my second year as an iGEMer, and I’m grateful to be back, charged and reloaded! </p>
  
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        <h3>César Ibrahym Rodríguez Fernández</h3>
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  <h2 align="center">Abstract</h2>
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        <p>I’m a 19 years old student of biotechnology engineering. Science has always been a part of me, as well as my love for american football and music, specially playing piano and guitar. Let’s see how this goes, I’m thankful to be in and motivated to be part of a great project! </p>
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  <p align="justify"> American and European Foulbrood are diseases affecting honey bee (<i>Apis mellifera</i>) larvae all around the world. The causal agents of these are two gram-positive bacteria: <i>Paenibacillus larvae</i> and <i>Melissococcus plutonius</i>. Nowadays, two techniques for the treatment of AFB and EFB are used: antibiotics and incineration of affected hives. The former promotes the development of antibiotic resistance in bacteria while the latter results unprofitable for beekeepers. The production of native bee antimicrobial peptides (AMPs) in <i>Escherichia coli</i> is proposed to treat <i>P. larvae</i> and <i>M. plutonius</i> infections. Defensin 1, abaecin, defensin 2, and apidaecin will each be expressed in a different culture. A 2⁴ factorial design will be used to identify the optimal AMP combination. The final product, AMPs with a specific packaging, will be available for beekeepers to apply in their beehives and inhibit the proliferation of pathogenic bacteria.</p>
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        <h3>Zazil Adriana Solís Saldívar</h3>
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  <p align="justify"> American and European Foulbrood are diseases that affect honey bee (Apis mellifera) larvae all around the world. The causal agents of these are two Gram-positive bacteria: <i> Paenibacillus larvae </i> and <i> Melissococcus plutonius </i>, respectively. <i> P. larvae </i> is an endospore-forming bacterium which infects larvae through nurse bees and consumes them until only a biomass of spores is left in the cell. <i> M. plutonius </i>, named after the Greek god of death, outcompetes larval honeybees for food and leaves them starving before “melting” them. </p>
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        <p>Hi there! I’m 22 years old, making me the mummy of the group, haha I know I’m hilarious. I’m about to start the 7th semester of my career which is Biotechnology Engineering. I am excited to develop our bee project and beeing part of the iGEM community. I enjoy learning about other countries and traditions so that is why I was a Rotary Youth Exchange student in Brazil 5 years ago. I love traveling, Shakira’s old songs, rainy days and FOOD. My first laboratory experiment consisted in creating José Pablo Rascón Pérez, a Palm tree who makes photosynthesis.
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  <p align="justify"> These two diseases cause enormous losses in the apiculture industry and contribute to the factors that threaten all bees. Nowadays, two techniques for the treatment of AFB and EFB are used: antibiotics and incineration of affected hives. There are harsh regulations on the use of antibiotics and doubly so when talking about exported goods. Antibiotics not only make the product harder to sell, but also promote the development of resistance in bacteria. Incineration is unprofitable for beekeepers and often requires extensive authorization processes. </p>
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        <h3>Pablo Espinoza Hernandez</h3>
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        <p>Hello there! I’m a 19 years old biotechnology engineering student. I just finished 2nd semester and i am excited for what is coming next. Curiosity has always been inside me and science is a great way to fulfill that hunger of me for new knowledge. I have a huge love for sci-fi, 90's Hip-Hop, Space exploration, SpaceX, traveling, new cultures, chemistry and i like to call myself a dreamer for a better tomorrow. I speak spanish as my native language, english as my second an currently learning to speak Brazilian portuguese “Boa tarde”. </p>
  
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  <p align="justify"> However, insects possess several antimicrobial peptides (AMPs) as part of their innate immune response. Native bee AMPs produced in <i> Escherichia coli </i> could be an effective alternative for the treatment of <i> P. larvae </i> and <i> M. plutonius  </i> infections. </p>
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        <h3>César Aarón Villalobos Díaz</h3>
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        <p>Twenty years of life have led me to study Biotechnological Engineering. Although I’d always rather stay home and play tabletop games on a rainy day, helping build a better world is the next best thing. #TeamCesar </p>
  
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  <p align="justify"> Defensin 1 and 2, abaecin, and apidaecin are suggested for this purpose. These AMPs possess different mechanisms of action. The defensins depolarize the membrane, open channels in it, and allow the efflux of potassium ions, which would either be compensated with contaminating cations or efflux of anions, destabilizing biochemical processes. There also is evidence that defensins stop the respiratory activity and reduce the level of ATPs present. Abaecin and apidaecin, on the other hand, stop protein synthesis through the interference of the 70S ribosome and inhibits DnaK activity. Apidaecin binds to LPS and disrupts the ABC transport system, as well. </p>
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        <h3>Ana Laura Ortega</h3>
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        <p>Hi! I’m eighteen years old and just about to start my third semester in Biotechnology Engineering. I have always loved nature and still get surprised every day with it; iGEM is a great opportunity to learn more about it. I love avocados, dancing and getting to know other countries and cultures, I really enjoy learning different languages. I’m glad to be part of this amazing team and wondering what this new experience will bring. </p>
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  <p align="justify"> The project’s first objective is to express these AMPs in a different bacterial culture each, with an inducible promoter. T7 RNA polymerase expression is regulated by the Lac operon, which is induced through the presence of IPTG. Therefore, the usage of T7 promoters allows us to induce AMP production. Proteins will be extracted through sonication of bacterial cultures and then isolated through a His-tag purification process. </p>
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  <p align="justify"> Once all four AMPs are purified, all possible combinations will be tested against <i> P. larvae </i> and <i> M. plutonius </i>. A 2⁴ factorial design will be used to evaluate individual effects, quantify interactions, and identify the optimal AMP combination. </p>
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  <p align="justify"> PLGA-microencapsulated AMPs would be added to bees’ diet so nurses can bring the AMPs to the infected larvae. This microencapsulated AMPs would be available for beekeepers to apply in their hives and thus inhibit the proliferation of the pathogenic bacteria. </p>
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Revision as of 15:18, 11 August 2018

Erwinions


José Pablo Rascón Pérez

I’m 21 years old, currently in my 7th semester as a student of biotechnology engineering. Some of the things I like are: playing basketball, graphic design, science, playing soccer, camping, fishing, exercising and being around the people that I love. This is my second year as an iGEMer, and I’m grateful to be back, charged and reloaded!

César Ibrahym Rodríguez Fernández

I’m a 19 years old student of biotechnology engineering. Science has always been a part of me, as well as my love for american football and music, specially playing piano and guitar. Let’s see how this goes, I’m thankful to be in and motivated to be part of a great project!

Zazil Adriana Solís Saldívar

Hi there! I’m 22 years old, making me the mummy of the group, haha I know I’m hilarious. I’m about to start the 7th semester of my career which is Biotechnology Engineering. I am excited to develop our bee project and beeing part of the iGEM community. I enjoy learning about other countries and traditions so that is why I was a Rotary Youth Exchange student in Brazil 5 years ago. I love traveling, Shakira’s old songs, rainy days and FOOD. My first laboratory experiment consisted in creating José Pablo Rascón Pérez, a Palm tree who makes photosynthesis.

Pablo Espinoza Hernandez

Hello there! I’m a 19 years old biotechnology engineering student. I just finished 2nd semester and i am excited for what is coming next. Curiosity has always been inside me and science is a great way to fulfill that hunger of me for new knowledge. I have a huge love for sci-fi, 90's Hip-Hop, Space exploration, SpaceX, traveling, new cultures, chemistry and i like to call myself a dreamer for a better tomorrow. I speak spanish as my native language, english as my second an currently learning to speak Brazilian portuguese “Boa tarde”.

César Aarón Villalobos Díaz

Twenty years of life have led me to study Biotechnological Engineering. Although I’d always rather stay home and play tabletop games on a rainy day, helping build a better world is the next best thing. #TeamCesar

Ana Laura Ortega

Hi! I’m eighteen years old and just about to start my third semester in Biotechnology Engineering. I have always loved nature and still get surprised every day with it; iGEM is a great opportunity to learn more about it. I love avocados, dancing and getting to know other countries and cultures, I really enjoy learning different languages. I’m glad to be part of this amazing team and wondering what this new experience will bring.