Difference between revisions of "Team:Tec-Chihuahua/Safety"

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     <div class="content">     
 
     <div class="content">     
 
         <div class="container">
 
         <div class="container">
   
+
 
 +
      <h2 align="center"><b>P</b>ersonal <b>P</b>rotective <b>E</b>quipment (PPE)</h2>
 +
      <p> When being inside the lab, we have to follow certain rules, these to diminish the dangers that working inside a lab might represent. The rules go as follows:
 +
<div class="row">
 +
      <div class="col-md-3">
 +
            <img src="https://static.igem.org/mediawiki/2018/4/49/T--Tec-Chihuahua--Bata.jpg" class="img-responsive team-img center-block" height="300" width="250"/>
 +
              <p align="justify"> We always wear lab coats, nitrile gloves and masks avoiding contact with dropplets, aerosols and other contaminants resulting from experiments. </p>
 +
      </div>
 +
      <div class="col-md-3">
 +
            <img src="https://static.igem.org/mediawiki/2018/0/01/T--Tec-Chihuahua--Locker.jpg" class="img-responsive team-img center-block" height="300" width="250"/>
 +
              <p align="justify"> Our personal belongings stay away from the lab when handling infectious materials. </p>
 +
      </div>
 +
      <div class="col-md-3">
 +
            <img src="">
 +
              <p align="justify"> The PPE should never leave the lab, unless it's appropriately packed. </p>
 +
      </div>
 +
      <div class="col-md-3">
 +
            <img src="https://static.igem.org/mediawiki/2018/5/50/T--Tec-Chihuahua--Disinfect.jpg" class="img-responsive team-img center-block" height="300" width="250"/>
 +
              <p align="justify"> We should always disinfect our hands with 70% ethanol, applying it before and after we've put on the gloves, as well as washing our hands when entering the lab and after leaving it. </p>
 +
</div>
 +
<div>
 +
      <h2 align="center"><b>B</b>iological <b>S</b>afety <b>C</b>abinets (BSC)</h2>
 +
      <p align="justify"> We have 3 BSC, they protect us from the microorganisms we work with, as well as protecting the samples from the contamination we or other external agents might cause. As a common protocol, every single time we are about to use one of these cabinets, we should turn on the UV light for 15 minutes minimum, clean our hands and the materials we will introduce with 70% ethanol. As a final step, when done using it, we should clean it and let the UV light sterilize it for another 15 minutes. </p>
 +
<div class="row">
 +
      <div class="col-md-4">
 +
            <img src="https://static.igem.org/mediawiki/2018/1/11/T--Tec-Chihuahua--Emma.jpg" class="img-responsive team-img center-block" height="300" width="250"/>
 +
      </div>
 +
      <div class="col-md-4">
 +
            <img src="https://static.igem.org/mediawiki/2018/f/f5/T--Tec-Chihuahua--Anne.jpg" class="img-responsive team-img center-block" height="300" width="250"/>
 +
      </div>
 +
      <div class="col-md-4">
 +
            <img src="">
 +
      </div>
 +
</div>
 +
<br>
 +
<div>
 +
      <h2 align="center">Sterile halo: Using burners to create sterile zones</h2>
 +
      <p align="justify"> We count with two types of burners, nevertheless, they are used when working in an open bench. Having a burner in the working area is excellent to create a sterile zone, this to prevent cross-contamination among the samples we are working with. </p>
 +
<div class="row">
 +
      <div class="col-md-6">
 +
            <img src="https://static.igem.org/mediawiki/2018/f/f5/T--Tec-Chihuahua--Mekerfisher.jpg" class="img-responsive team-img center-block" height="300" width="250"/>
 +
              <p align="center"><b>Meker-fisher burner</b></p>
 +
      </div>
 +
      <div class="col-md-6">
 +
            <img src="">
 +
              <p align="center"><b>Bunsen burner</b></p>
 +
      </div>
 +
</div>
 +
      <h2 align="center">Lab Access and Waste Management</h2>
 +
      <p align="justify"> The labs we work in are categorized as Containment Level 2 Laboratories. They count with lockable doors. These labs are for Biotechnology alumni and teachers exclusive use. </p>
 +
      <p align="justify"> Usually we have to dispose of four types of wastes. They should be appropriately disposed in order to avoid every possible accident a confusion might cause. Sharp objects go in a thick red plastic bottle, biological wastes, such as fluids and organic tissues go in a yellow hermetic bottle. The solid wastes should be disposed in a yellow bag and all of the other materials that were in contact with bacteria or infectious agents, in general, go in a red bag. </p>
 +
<div class="row">
 +
      <div class="col-md-3">
 +
            <img src="">
 +
              <p align="center"><b>Red bottle for sharp objects</b></p>
 +
      </div>
 +
      <div class="col-md-3">
 +
            <img src="">
 +
              <p align="center"><b>Yellow bottle for non-solid biological wastes</b></p>
 +
      </div>
 +
      <div class="col-md-3">
 +
            <img src="">
 +
              <p align="center"><b>Yellow bag for solid biological wastes</b></p>
 +
      </div>
 +
      <div class="col-md-3">
 +
            <img src="">
 +
              <p align="center"><b>Red bag for infected materials</b></p>
 +
      </div>
 +
</div>
 +
      <h2 align="center"><i>Paenibacillus larvae</i> & <i>Melissococcus plutonius </i></h2>
 +
      <p></p>
 +
 
 
<div class="a">
 
<div class="a">
 
       <h2 align="center"> Safety Courses</h2>
 
       <h2 align="center"> Safety Courses</h2>
 
       <p align="justify"> Before starting any of the lab work, we completed courses provided by the Public Health Agency of Canada web page. In these courses, the whole team learned about the proper way to work in a Physical Containment Laboratory Level 2. All of the courses certified below were comprehended by the team to avoid any mistakes made inside the lab, preventing at the same time, accidents that could endanger someone. </p>
 
       <p align="justify"> Before starting any of the lab work, we completed courses provided by the Public Health Agency of Canada web page. In these courses, the whole team learned about the proper way to work in a Physical Containment Laboratory Level 2. All of the courses certified below were comprehended by the team to avoid any mistakes made inside the lab, preventing at the same time, accidents that could endanger someone. </p>
</div>
 
  
 
<div class="row">
 
<div class="row">
Line 87: Line 157:
 
</div>
 
</div>
 
</div>
 
</div>
      <h2 align="center"><b>P</b>ersonal <b>P</b>rotective <b>E</b>quipment (PPE)</h2>
 
      <p> When being inside the lab, we have to follow certain rules, these to diminish the dangers that working inside a lab might represent. The rules go as follows:
 
<div class="row">
 
      <div class="col-md-3">
 
            <img src="https://static.igem.org/mediawiki/2018/4/49/T--Tec-Chihuahua--Bata.jpg" class="img-responsive team-img center-block" height="300" width="250"/>
 
              <p align="justify"> We always wear lab coats, nitrile gloves and masks avoiding contact with dropplets, aerosols and other contaminants resulting from experiments. </p>
 
      </div>
 
      <div class="col-md-3">
 
            <img src="https://static.igem.org/mediawiki/2018/0/01/T--Tec-Chihuahua--Locker.jpg" class="img-responsive team-img center-block" height="300" width="250"/>
 
              <p align="justify"> Our personal belongings stay away from the lab when handling infectious materials. </p>
 
      </div>
 
      <div class="col-md-3">
 
            <img src="">
 
              <p align="justify"> The PPE should never leave the lab, unless it's appropriately packed. </p>
 
      </div>
 
      <div class="col-md-3">
 
            <img src="https://static.igem.org/mediawiki/2018/5/50/T--Tec-Chihuahua--Disinfect.jpg" class="img-responsive team-img center-block" height="300" width="250"/>
 
              <p align="justify"> We should always disinfect our hands with 70% ethanol, applying it before and after we've put on the gloves, as well as washing our hands when entering the lab and after leaving it. </p>
 
</div>
 
<div>
 
      <h2 align="center"><b>B</b>iological <b>S</b>afety <b>C</b>abinets (BSC)</h2>
 
      <p align="justify"> We have 3 BSC, they protect us from the microorganisms we work with, as well as protecting the samples from the contamination we or other external agents might cause. As a common protocol, every single time we are about to use one of these cabinets, we should turn on the UV light for 15 minutes minimum, clean our hands and the materials we will introduce with 70% ethanol. As a final step, when done using it, we should clean it and let the UV light sterilize it for another 15 minutes. </p>
 
<div class="row">
 
      <div class="col-md-4">
 
            <img src="https://static.igem.org/mediawiki/2018/1/11/T--Tec-Chihuahua--Emma.jpg" class="img-responsive team-img center-block" height="300" width="250"/>
 
      </div>
 
      <div class="col-md-4">
 
            <img src="https://static.igem.org/mediawiki/2018/f/f5/T--Tec-Chihuahua--Anne.jpg" class="img-responsive team-img center-block" height="300" width="250"/>
 
      </div>
 
      <div class="col-md-4">
 
            <img src="">
 
      </div>
 
 
</div>
 
</div>
<br>
 
<div>
 
      <h2 align="center">Sterile halo: Using burners to create sterile zones</h2>
 
      <p align="justify"> We count with two types of burners, nevertheless, they are used when working in an open bench. Having a burner in the working area is excellent to create a sterile zone, this to prevent cross-contamination among the samples we are working with. </p>
 
<div class="row">
 
      <div class="col-md-6">
 
            <img src="https://static.igem.org/mediawiki/2018/f/f5/T--Tec-Chihuahua--Mekerfisher.jpg" class="img-responsive team-img center-block" height="300" width="250"/>
 
              <p align="center"><b>Meker-fisher burner</b></p>
 
      </div>
 
      <div class="col-md-6">
 
            <img src="">
 
              <p align="center"><b>Bunsen burner</b></p>
 
      </div>
 
</div>
 
      <h2 align="center">Lab Access and Waste Management</h2>
 
      <p align="justify"> The labs we work in are categorized as Containment Level 2 Laboratories. They count with lockable doors. These labs are for Biotechnology alumni and teachers exclusive use. </p>
 
      <p align="justify"> Usually we have to dispose of four types of wastes. They should be appropriately disposed in order to avoid every possible accident a confusion might cause. Sharp objects go in a thick red plastic bottle, biological wastes, such as fluids and organic tissues go in a yellow hermetic bottle. The solid wastes should be disposed in a yellow bag and all of the other materials that were in contact with bacteria or infectious agents, in general, go in a red bag. </p>
 
<div class="row">
 
      <div class="col-md-3">
 
            <img src="">
 
              <p align="center"><b>Red bottle for sharp objects</b></p>
 
      </div>
 
      <div class="col-md-3">
 
            <img src="">
 
              <p align="center"><b>Yellow bottle for non-solid biological wastes</b></p>
 
      </div>
 
      <div class="col-md-3">
 
            <img src="">
 
              <p align="center"><b>Yellow bag for solid biological wastes</b></p>
 
      </div>
 
      <div class="col-md-3">
 
            <img src="">
 
              <p align="center"><b>Red bag for infected materials</b></p>
 
      </div>
 
</div>
 
      <h2 align="center"><i>Paenibacillus larvae</i> & <i>Melissococcus plutonius </i>
 
      <p></p>
 
 
</div>
 
</div>
 
</div>
 
</div>

Revision as of 21:22, 14 August 2018

] Erwinions



Personal Protective Equipment (PPE)

When being inside the lab, we have to follow certain rules, these to diminish the dangers that working inside a lab might represent. The rules go as follows:

We always wear lab coats, nitrile gloves and masks avoiding contact with dropplets, aerosols and other contaminants resulting from experiments.

Our personal belongings stay away from the lab when handling infectious materials.

The PPE should never leave the lab, unless it's appropriately packed.

We should always disinfect our hands with 70% ethanol, applying it before and after we've put on the gloves, as well as washing our hands when entering the lab and after leaving it.

Biological Safety Cabinets (BSC)

We have 3 BSC, they protect us from the microorganisms we work with, as well as protecting the samples from the contamination we or other external agents might cause. As a common protocol, every single time we are about to use one of these cabinets, we should turn on the UV light for 15 minutes minimum, clean our hands and the materials we will introduce with 70% ethanol. As a final step, when done using it, we should clean it and let the UV light sterilize it for another 15 minutes.


Sterile halo: Using burners to create sterile zones

We count with two types of burners, nevertheless, they are used when working in an open bench. Having a burner in the working area is excellent to create a sterile zone, this to prevent cross-contamination among the samples we are working with.

Meker-fisher burner

Bunsen burner

Lab Access and Waste Management

The labs we work in are categorized as Containment Level 2 Laboratories. They count with lockable doors. These labs are for Biotechnology alumni and teachers exclusive use.

Usually we have to dispose of four types of wastes. They should be appropriately disposed in order to avoid every possible accident a confusion might cause. Sharp objects go in a thick red plastic bottle, biological wastes, such as fluids and organic tissues go in a yellow hermetic bottle. The solid wastes should be disposed in a yellow bag and all of the other materials that were in contact with bacteria or infectious agents, in general, go in a red bag.

Red bottle for sharp objects

Yellow bottle for non-solid biological wastes

Yellow bag for solid biological wastes

Red bag for infected materials

Paenibacillus larvae & Melissococcus plutonius

Safety Courses

Before starting any of the lab work, we completed courses provided by the Public Health Agency of Canada web page. In these courses, the whole team learned about the proper way to work in a Physical Containment Laboratory Level 2. All of the courses certified below were comprehended by the team to avoid any mistakes made inside the lab, preventing at the same time, accidents that could endanger someone.