Revision as of 11:19, 17 August 2018

In our project we are developing a new technology platform based on the knowledge of the role of sRNAs in bacteria to control antibiotic resistance. We will use E.coli K12 to test our strategies by engineering libraries of sRNA scaffolds to modulate gene expression patents in relation to the external environmental factors.

What are we Doing?

We are developing a new technology platform based on 3'UTR sequence characterisation to control soluble protein expression in E.coli.

We will use E.coli K12 to test our "Proof of Concept" Principle by engineering 3'UTR sequences (Sharma et al, 2011) to interact with either cytosolic proteins or 5'UTR sequences of our gene of interest: trxA and gor. Down regulation of these genes will be quantified by assessing quantitatively GFP signal. Efficiency of down regulation of genes will be quantified by assessing reaction kinetics of Alkaline Phosphatase (AP).

FULL PROJECT DESCRIPTION

See Our Work

Education and Outreach

You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.

When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)

Lab Work

We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!

Social Media Engagement

On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!

Use WikiTools - Edit in the black menu bar to edit this page

EDIT PAGE

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

State your accomplishments! Tell people what you have achieved from the start.
Be clear about what you are doing and how you plan to do this.
You have a global audience! Consider the different backgrounds that your users come from.
Make sure information is easy to find; nothing should be more than 3 clicks away.
Avoid using very small fonts and low contrast colors; information should be easy to read.
Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2018 calendar
Have lots of fun!

Inspiration

You can also view other team wikis for inspiration! Here are some examples:

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 We will use E.coli K12 to test our "Proof of Concept" Principle by engineering 3'UTR sequences (Sharma et al, 2011) to interact with either cytosolic proteins or 5'UTR sequences of our gene of interest: trxA and gor. Down regulation of these genes will be quantified by assessing quantitatively GFP signal. Efficiency of down regulation of genes will be quantified by assessing reaction kinetics of Alkaline Phosphatase (AP).</p>
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 FULL PROJECT DESCRIPTION</a>
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