Team:NTNU Trondheim/Improve

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The RRvT’s strong brightness and it’s low sensitivity of acidic environment makes the new biobrick a clear improvemt of the existing red fluorescent protein.

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Gold medal criterion two, and best composite part award:

To improve a previous part, we decided to change the sequence of the red fluorescent protein coding device BBa_J04450. This was done by replacing the RFP sequence with the sequence for the RRvT. RRvT is a red fluorescent protein with a much higher brightness, and it can be used when the concentration of the inducer is to low to activate the BBa_J04450 biobrick. The RRvT has a very low acid sensitivity which can be useful in many situations. The advantages of RRvT’s strong brightness and it’s low sensitivity of acidic environment makes this protein better than the existing red fluorescent protein.

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