Attributions

iGEM Leiden 2018 would like to acknowledge the invaluable contributions of all those who have helped to advance our project over the past months. Without their support it would not have been possible to successfully perform our experiments in the laboratory, promote or fundraise our project. Not only did they help in making this iGEM project possible, they have also contributed to making this experience incredibly fun! This page is dedicated to all these wonderful people and organisations.

Our special thanks goes to:

Principle Investigators

Dennis Claessen
Han de Winde

Advisors

Charlotte van de Velde
Sjoerd Seekles
Vincent Bakker

General Support

Laura van Achteren
For her help in creating the invoices for our sponsors
Hestia Tamboer & Sandra Jansen
For their tremendous dedication and help in booking our flights to Boston
Kick Moors
For his guidance in the team role formation process
Anne Maren Kruse
For her efforts in securing working space and funding at Leiden University
Gerda Lamers
For allowing us to use the DinoLite ocular for making microscopy images and for helping us with the microscopy experiments

Experimental Support

Kees Koops
For his help in setting up our lab space and his readiness to help us in all our (minor) crises
Lizah van der Aart
For her invaluable advice on the overlay technique as well as her efforts in 3D printing keychains for our crowdfunding campaign
Ellen de Waal & Ilja Bekker
For their help with ordering our lab materials from various suppliers
Thomas Bakkum
For generously allowing us to use the FACS machine of his department
Paul van Heusden
For his patient explanation of the FACS machine
Gerben Voshol
For his advice on fixating bacteria for FACS analysis
iGEM Groningen 2018
For providing us with the pNZ8040 and pMK4 plasmids for our bacillus subtilis subproject
iGEM Delft 2018
For providing us with the transformed E.coli DH5α for the InterLab

Fundraising

Annette Heijn
For supporting us before and during our crowdfunding campaign, for her indispensable advice and her continuous assistance
Milou van Leeuwen
For her dedication in making our crowdfunding video absolutely perfect
Corine Hendriks
For her article about our project and crowdfunding campaign on the university website
Wendy Persson
For her general assistance during the crowdfunding campaign and for troubleshooting when required
Roy Zitman (PLNT)
For providing us with contacts within Leiden and leads on where to secure funding for our project
The Science Meets Business Foundation, in specific:
Max Green, Max van 't Hof, Vincent van der Wel and Coen Breedveld
For providing the team with general advise regarding fundraising, project management and crowdfunding

Societal Outreach

Danny Rozen
For allowing us to present our project at This Weeks Discovery, a university platform where scientists gather to present their current research
Federico Muffatto
For providing us with the M. roseus and M. luteus strains to use for bioart in our societal outreach
Margot Duesmann
For allowing us to organise a workshop during Night at the Museums in Leiden
Frédérique de Paus
For coordinating our team’s involvement in Dutch festival Werfpop

Team Contributions

This project was designed, organised and executed by members of our team. The initial idea for our project was the result of an internship completed by our Science manager Marjolein Crooijmans under our PI Dennis Claessen. Although everyone had their own role to play within the team, we helped each other in all project aspects. We credit this willingness to help each other as the most important factor in our successes.

Experimental Work

In our lab we benefited from a very free working atmosphere, and even though people were assigned to a specific subproject we all pitched in and helped each other where needed.

Before our project started, Marjolein worked on isolating our first 30 stress promoters and coupling them to GFP constructs to enable testing.

Charlotte de Ceuninck van Capelle and Germaine Aalderink
Charlotte and Germaine were responsible for the selection of the bacillus subtilis promoters as well as transforming bacillus with a chromoprotein.
Daphne van den Homberg and Mees Fox
Daphne created all our new promoter constructs using PCR. As a team, Daphne and Mees focussed on coupling these new promoters to GFP.
Maaike de Jong and Lotte Weel
Maaike and Lotte occupied themselves with the construction of our composite plasmid.
Tim de Jong and Carli Koster
Tim and Carli were responsible for the validation of our system using fluorescence-activated cell sorting.
Jazzy de Waard and Marijke Grundeken
As antibiotics are traditionally discovered with the overlay techniques using streptomyces and E.coli, Jazzy and Marijke learned to perform this technique as well as disk diffusion.
Laurens ter Haar and Marjolein Crooijmans
Laurens and Marjolein were concerned with enhancing our system so we could obtain a clear read-out. To this end they used T7.

Fundraising and Finances

Tim de Jong and Jazzy de Waard functioned as our Fundraising team and were mainly responsible for approaching sponsors and managing our crowdfunding campaign. Our treasurer Maaike de Jong safeguarded our finances and managed our budget with occasional help from Charlotte de Ceuninck van Capelle.

Design

Our secretary and design manager Daphne van den Homberg designed all of our promotion materials, including flyers, banners, postcards et cetera with help from Laurens ter Haar and Chiel van Amstel.

Collaborations

Carli Koster was responsible for the countless collaborations that we did with teams from all around the world, such as the postcards from team Düsseldorf, the Instagram Challenge for July and Moves Like iGEM amongst others. Charlotte de Ceuninck van Capelle managed the collaboration between Leiden and Rotterdam HR as well as the Dutch Meet-Up and BBQ.

Programming and Statistics

All of our wiki programming and statistics were performed by Chiel van Amstel.

Outreach and Social Media

Our PR team, Carli Koster and Marijke Grundeken, managed all of our social media accounts and our monthly newsletter, while Mees Fox arranged for us to be present at local events to give workshops and talks about our project.

InterLab

Charlotte de Ceuninck van Capelle arranged for iGEM Leiden to be able to use the transformed InterLab strains from team Delft. All InterLab experiments were performed by Chiel van Amstel and Jazzy de Waard.

Lab Safety

Naturally, all of our team members took lab safety extremely seriously. However, Germaine Aalderink was responsible for arranging the GMO course everyone needed to complete in order to work in the ML-1 lab and for completing our safety form.

Presentations

Laurens ter Haar presented our project at events like This Week’s Discovery and the Science Meets Business Cafe.

Wiki

As our programming manager Chiel programmed the entire wiki. All team members contributed to writing the wiki but the final content was managed by Laurens ter Haar.

Team:Leiden/Attributions