Team:Kyoto/Notebook

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Notebook

July
		1	2	3	4	5
6	7	8	9	10	11	12
13	14	15	16	17	18	19
20	21	22	23	24	25	26
27	28	29	30	31

August
		1	2	3	4	5
6	7	8	9	10	11	12
13	14	15	16	17	18	19
20	21	22	23	24	25	26
27	28	29	30	31

September
		1	2	3	4	5
6	7	8	9	10	11	12
13	14	15	16	17	18	19
20	21	22	23	24	25	26
27	28	29	30	31

October
		1	2	3	4	5
6	7	8	9	10	11	12
13	14	15	16	17	18	19
20	21	22	23	24	25	26
27	28	29	30	31

7/3

Restriction Enzyme Digestion

Tong, Tamukai

component	volume(&microl)
BSA	0.5
buffer(10xH)	5
EcoR1	0.5
Pst1	0.5
Dpn1	0.5
dH2O	18

add 4&microL linearized plasmid backbone (25ng/&microL)
add 4&microL Enzyme Master Mix
digest 37℃, 30min
add 8&microL NaOAc (pH5.2,3M)
add 16&microL 99.5% EtOh
Vortex 2sec
add 184&microL 99.5% EtOH
add 72&microL DW

7/5

PCR

Tong, Nambu, Shimazoe

sample	Primer Forward	volume (ul)	Primer Reverse	volume (ul)	template DNA	volume (ul)	Q5 HF 2xMasterMix (ul)	DW (ul)
1	Z007	1.25	Z009	1.25	Yeast genome	1.0	1.0	9.0
2	Z008	1.25	Z011	1.25	Yeast genome	1.0	1.0	9.0
3	Z010	1.25	Z012	1.25	Yeast genome	1.0	1.0	9.0
4	Z007	1.25	Z014	1.25	Yeast genome	1.0	1.0	9.0
5	Z013	1.25	Z016	1.25	Yeast genome	1.0	1.0	9.0
6	Z015	1.25	Z012	1.25	Yeast genome	1.0	1.0	9.0