Team Göttingen
iGEM 2018
Glyphosate on my plate?
Background
The discovery of the weedkiller glyphosate
Glyphosate (N-(phosphonomethyl)glycine) was first synthesized by the chemist Dr. Henri Martin in 1950, while working for the Swiss pharmaceutical company Cilag, which was founded in 1936 in Schaffhausen. Unfortunately (fortunately?), Dr. Martin did find out that glyphosate may serve as a very efficient herbicide (see below). About 20 years later, the American chemist Dr. John E. Franz who was working for the American company Monsanto (recently bought by Bayer) observed that glyphosate specifically inhibits the 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase in plants, fungi, bacteria and archaea (Figure 1) (1-4). The EPSP synthase generates the precursor for the de novo synthesis of aromatic amino acids tryptophan, tyrosine and phenylalanine (5). Therefore, inhibition of the EPSP synthase by glyphosate results in the depletion of the cellular levels of aromatic amino acids and death of the organism that is treated with the weedkiller (Figure 1) (1,6-8).
Figure 1. Glyphosate that is present in Roundup specifically inhibits the 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase (PDBid: 2QFU), which converts shipmate-3-phosphate (S3P) and phosphoenolpyruvate (PEP) to EPSP. A drop in the cellular levels of the precursor for aromatic amino acid synthesis causes death of the organism that has been treated with Roundup.
The usage of glyphosate
Glyphosate is resistant to chemical hydrolysis, thermal decomposition and photolysis due to a stable C-P bond (9). Moreover, due to the fact that glyphosate is toxicologically safe and that transgenic, glyphosate-resistant crops have been introduced by Monsanto, the herbicide has become the dominant weedkiller worldwide (10-15). The production and usage rate of Glyphosate increasing up to this day (Figure 2). Moreover, given the fact that genetically modified crops that produce glyphosate-insensitive EPSP synthases are tolerated in the united states, glyphosate is the most used herbicide in this country (13).
Figure 2. Glyphosate usage over the last two decades. It has been estimated that the production of glyphosate is increasing with a rate of about 40 tons per year. Adapted from Benbrook (13).
The problem of glyphosate-resistant crops
Due to the intensive application of glyphosate in agriculture, several plant species have become resistant against the herbicide. Please check out the webpage http://www.weedscience.org, which is reporting of herbicide resistant weeds globally. The international survey of herbicide resistant weeds is a collaborative effort between weed scientists in over 80 countries. The main aim of this collaborative effort is to maintain scientific accuracy in the reporting of herbicide resistant weeds globally. The collaborative effort is supported by government, academic, and industry weed scientists worldwide. Moreover, the project is funded by the Global Herbicide Resistance Action Committee and CropLife International. The development of glyphosate resistance is a severe problem in agriculture because increasing amounts of the herbicide have to be applied in order to kill weed. Unfortunately, the increased usage of glyphosate may negatively affect the biodiversity because the weedkiller does not discriminate between weed and other plants that serve as a food source for insects like honey bees. Since honey bees are very important for pollination a decrease of the honey bee population has a strong negative impact on plant reproduction, which also affects the fruit yield. Moreover, since crops as well as weed co-evolve with glyphosate over time, the development of herbicide resistance will never stop.
Other applications for glyphosate
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The glyphosate controversy
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References
- Amrhein et al. (1983) FEBS Lett. 157: 191-196.
- Comai et al. (1983) Science 221: 370-371.
- Schulz et al. (1984) Arch. Microbiol. 137: 121-123
- Steinrücken & Amrhein (1980) Biochem. Biophys. Res. Commun. 94: 1207-1212.
- Herrmann & Weaver (1999) Annu. Rev. Plant. Physiol. Plant. Mol. Biol. 50: 473-503.
- Fischer et al. (1986) J. Bacteriol. 168: 1147-1154
- Gresshoff (1979) Aust. J. Plant. Physiol. 6: 177-185.
- Majumder et al. (1995) Eur. J. Biochem. 229: 99-106.
- Kononova & Nesmeyanova (2002) Biochemistry (Mosc) 67: 184-195.
- Li & Long (1988) Fundam. Appl. Toxicol. 10: 537-546.
- Duke & Powles (2008) Pest. Manag. Sci. 64: 319-325.
- Arjó et al. (2013) Transgenic Res. 22: 255-267.
- Benbrook (2016) Environ. Sci. Eur. 28: 3.
- Mesnage & Antoniou (2017) Front. Public. Health. 5: 316.
- Tincher et al. (2017) G3 (Bethesda). 7: 3331-3335.