Team:Munich/deletioncasette.html
Contents
5/28
Transforming E. Coli DH5a to amplify pKD3 for pRED/ET engineering
| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | we also inoculated LB media |
| Results: | no colonies, no growth
We decided to use a resistance cassette from pSB1C3 without FRT sites |
5/29
Amplifying a selection cassette from pSB1C3
| Participants: | Eni |
| Protocol: | PCR |
| Notes: | Primer with 50bp homology regions, Ta: 67°C, Elongation: 45s |
| Results: | no results |