Team:Munich/deletioncasette2.html
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Creating a selection cassette from pSB1C3
| Participants: | Dominic |
| Protocol: | PCR, Agarose Gel, Gelextraction |
| Notes: | Knockouts of both msb-B, RecBCD
Primer with 50bp homology regions, Ta: 62°C, Elongation: 45s Expected: 1,1kb |
| Results: | no colonies |