Team:Mingdao/Description

Design

Thioredoxin fusion system

To create a reporter system, we constructed a GFP expression vector. We amplified a constitutive promoter from Drosophila actin 5c gene and an eukaryotic poly A signal by PCR from pAc5.1 vector. The resulting DNA fragments were assembled with a BioBrick existing part of GFP to generate the reporter vector of Ac5-GFP-polyA / pSB1C3 (K2543004).

Reference

Antidote

Test Strip

Kit