Team:TUDelft/Wetlab/Protocols

Text to write to introduce the protocols

Colony PCR

1. Under sterile conditions, pick a colony and dilute it in 10 µL of milli-Q water.
   NOTE: After you pick the colony, it cannot be used again. It is therefore recommended to make a 'back-up'-plate where you grow the colonies again. This plate should be incubated overnight at 37 °C.
2. Incubate at 90 °C for 10 min.
   NOTE: Instead of separate 'cooking' of the cells before the PCR, this step can be incorporated in the PCR program. The initial denaturation step at 98 °C should then be prolonged to 5 minutes (300 seconds).
3. Prepare the GoTaq master mix for all the samples in a single 1.5 mL tube
   NOTE: mind pipetting errors so prepare at little bit more master mix!. >
   For one sample: