Team:JNFLS/Design

Design

In the very beginning of our project, we brainstormed about the detection measures for HCV. The current detection method used in all blood centers in Shandong province is ELISA-based HCV antibody detection, which caused the awkward situation that not even a single positive result was gained in the last decade. HCV antibody detection will be the past, but what the future will be? ELISA-based antigen detection? No, the detection must be sufficiently sensitive, what if the amount of virus in the blood is too low? Nucleic acid detection? No, it shares the same drawback as the previous method; also, RNA is way easier to be degraded. In the end, the works of Zhang et al and Zhou inspired us. We eventually designed a biosensor based on aptamer and rolling circle amplification.

1. Li Shengtao. Purification of expression of HCV "truncated" core protein (HCV Core125) and preparation of antibodies [D]. Kunming university of technology,2012.
2. Wu Xianbo. Cloning, expression and purification and antigenicity analysis of HCV core antigen gene fragments [D]. First military medical university of the people's liberation army,2003.
3. Zhang Songbai, Zheng Liying, Hu xia, Shen Guangyu, Liu Xuewen, Shen Guoli, Yu Ruqin. High sensitivity detection of thrombin by fluorescent adaptor sensor based on competitive trigger rolling ring amplification [J]. Analytical chemistry,2015,43(11):1688-1694.
4. Zhou hui. Study on nucleic acid aptamer sensor based on signal amplification triggered by competitive mechanism [D]. Hunan university,2009.