Team:CPU CHINA/Notebook/Log
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Early Stage
As is shown in Human Practice (Silver)（超链接HPSilver）, we China Pharmaceutical University has a club for synthetic biology and iGEM. This year’s team started from Kexin Cao, Yunge Lou, Junqing Zhang, Shuyao Zhou and Huandi Xu. These 5 have long been club members of CPU Bio-X Club.They witnessed the success of their <a href="https://2017.igem.org/Team:CPU_CHINA">senior schoolmates</a>, and was determined and ready for this year’s competition. After intense group study and brainstorming for 4 months, Shuyao Zhou’s scheme of condition RNA interference (though very rough compared with today’s whole project) was themostfeasible one, and was approved by our PI Prof. Tian.
12/2-12/9/2017
Site-directed mutagenesis of hTERT and HULC promoter sequences.(Design) Contemplating on what (mathematical) model would apply.(Model) Literature research: the significance of MAP4K4 gene and how the tumor would react after it is silenced, how theinhibitory strand could be transcripted.(Design) Write an experiment protocol: subcutaneous tumorigenesis in nude mice.(Lab) Select a proper RdRP.(Design)
12/10-12/12/2017
Select aligand-inducible promoter/RdRP inhibitors.(Design) Determine the sequence ofhTERTandHULC promoter.(Design) Literature research: the functioning mechanism of MAP4K4 in hepatocellular carcinoma.(Design) Analytical method of HCV RdRP.(Lab) Design a double-ncRNA-encoding vector.(Design) Select RdRP and ncRNA sequence.(Design)
1/21-1/26/2018
Design a miRNA spongewhich couldtarget Sil-MAP4K4.(Design) Research on vectors.(Design) Construct CMV-tet-tTA system.(Design) Design the pSil-MAP4K4 and inhibitory strand.(Design) Write aproposal about the whole scheme, including the principle, technical route and research contents, for our PI to systematically evaluate.(Design)
1/27-1/28/2018
Make a budget for experimental materials and reagents.(Lab)
1/29-1/31/2018
Plan the whole experimenting process.(Lab) Prepare a schematic diagram.(Design)
Winter vacation
sign up and pay the fees.
3/4-3/9/2018
Determine the exact sequence of the miRNA sponge.(Design) Combine the HULC promoter and pSil-MAP4K4 sequence.(Design) Call for the synthesisof parts involved in Plasmid 1（超链接） by biotechnology company.(Experiment)
3/10-3/20/2018
Determine the exact sequence for parts involved inPlasmid 2（超链接）.(Design) Brainstorming a brand name for ourproject.(Miscellaneous) Contemplating on what (mathematical) model couldbe applied to non-experimental use in the whole project. (Model) Recruit new members. Nannan Liu and Wanli Zhang joinedour team!
3/21-3/26/2018
Hongyu Wang, Yiyang Zhu, Peng Sun and Ying Miao joined us! Ponder the novelty of the project.(Design)
3/27-4/1/2018
Design a scheme for Human Practice.(HP)
4/1-4/7/2018
Determine the project's name.(Miscellaneous) Prepare for cross-school talks.(HP) Finish the presentation for the funding from“Training Program of Innovation and Entrepreneurship of College Students in China Pharmaceutical University”.(HP) Research on the general evaluation method for acancer treatment protocol.(HP)
4/14-4/22/2018
Investigate the pharmacology property of two first-line treatment macromolecular drugs, both delivered by lipidosome.(HP) Design the team uniform, working card, team logo and a presentation scroll.(Miscellaneous) Look for sponsorship.(Sponsor)
4/23-4/28/2018
Draw up a plan of interviewing experts in different but relative fields.(HP) Validate the model structure and design a questionnaire about the costs and side reaction of therapies for HCC.(Model)
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To be continued.
