Team:Munich/thisisatest.html
5/7
Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | pkD3 contains resistance cassettes with FRT-sites for pRED engineering incubate pRED at 30°C because of temperature sensitive promoter
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| Results: | no colonies |
5/18
Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | inoculate pRED at 30°C because of temperature sensitive promoter |
| Results: | no colonies |
5/24
Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | inoculate pRED at 30°C because of temperature sensitive promoter, pKD3 contains resistance cassette flanked by FRT sites |
| Results: | no colonies |
5/25
Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | chem. Trafo |
| Notes: | pKD3 contains resistance cassette flanked by FRT sites |
| Results: | no colonies |
5/26
DNA preparation for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | mini prep |
| Notes: | because pRED/ET could not be transformed, we got readily transformed cells from PD Dr. Jürgen Lassak from the LMU |
| Results: | pRED/ET: 37,5 ng/ul
pNPTS138-R6KT: 60ng/ul |