Team:Munich/chibiobrick1.html
6/4
Digestion of Chi6 with XbaI, SpeI
| Participants: | Dominic |
| Protocol: | restriction digest |
| Notes: | XbaI, SpeI |
6/4
Ligation of Chi6 with pSB1C3
| Participants: | Dominic |
| Protocol: | Ligation |
6/4
Transforming DH5a with pSB1C3_Chi6
| Participants: | Dominic |
| Protocol: | chem trafo |
| Notes: | |
| Results: | colonies |
6/5
DNA Mini preparation from E. Coli Dh5a pSB1C3_Chi6
| Participants: | Dominic |
| Protocol: | mini prep |
| Notes: | |
| Results: | concentration |
6/5
Sequence pSB1C3_Chi6
| Participants: | Dominic |
| Protocol: | meh |
| Notes: | |
| Results: | no sequence for the given primers: contamination |
6/6
Redo assembly of Chi6 using A3 assembly
| Participants: | Dominic |
| Protocol: | restriction digest, PCR purification |
| Notes: | XbaI, SpeI |
| Results: |
6/26
Test PCR for pSB1C3_Chi6
| Participants: | Dominic |
| Protocol: | PCR, Agarose gel |
| Notes: | |
| Results: | No bands on the gel. colonies on the plate resulted from contamination |