Team:SCAU-China/Interlab
The goal of the iGEM InterLab Study is reliable and repeatable measurement which is a key component to synthetic biology. We took part in the 5th International InterLab Measurement Study and the big question is
“Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD? ”
Parts
Interlab test devices used, as set by the iGEM measurement committee
| Device | Part Number | Location |
| Negative control | BBa_R0040 | Well 2D |
| Positive control | BBa_I20270 | Well 2B |
| Test Device 1 | BBa_J364000 | Well 2F |
| Test Device 2 | BBa_J364001 | Well 2H |
| Test Device 3 | BBa_J364002 | Well 2J |
| Test Device 4 | BBa_J364007 | Well 2L |
| Test Device 5 | BBa_J364008 | Well 2N |
| Test Device 6 | BBa_J364009 | Well 2P |
the preparation of competent cells(Escherichia coli strain DH5α) and their transformation according to the cell measurement protocol. Meanwile, we followed the Calibration Protocols supplied by the iGEM HQ to generate OD600 Reference point, Particle Standard Curve and Fluorescence standard curve to calibrate the plate reader(96 well plate) and then measured both the fluorescence and absorbance of our samples in 0-hour and 6-hour.
We used the set protocol that we were given so as to ensure everyone was doing the same thing. These can be found at this
School's name:SCAU
Member's name:SCAU
Designed by:SCAU