Basic Parts

BBa_K2719000: GST Coding site

Glutathione S-transferase (GST) is a peptide derived from Schistosoma japonicum, in which a target protein may be fused on the N-terminal. This fusion allows the purification of the target protein using glutathione affinity and maximize the presence of the protein in soluble state, rather than on inclusion bodies. This tag may be later removed by adding a protease recognition site between GST and the target protein (Harper & Speicher, 2013).

BBa_K2719002: GST-Tenascin Domain V Fusion Protein

This part is the fusion between both proteins and that helps us to simplify tenascin purification. GST has tenascin fused on the N-terminal. For more information see BBa_K2719001 and BBa_K2719003.

BBa_K2719004: Tenascin Fibronectin Domains

Coding sequence from tenascin fibronectin domains I-V have the property of interacting with the proliferation of fibroblast, but the domain with the main characteristic previously mentioned is domain V (see part BBa_K2719001).

BBa_K2719008: Leptin coding site.

This is one of the most important parts because is the growth factor that we are using for accelerating the process of skin regeneration. Is a protein that is found in adipocytes cells on many living beings and it has been discovered that it has a regenerating property, because it has been found in many wound repair processes. It has been shown that it increases glucose intake speeding up metabolism, thus, accelerating cell proliferation.

A basic part is a functional unit of DNA that cannot be subdivided into smaller component parts. BBa_R0051 is an example of a basic part, a promoter regulated by lambda cl.

Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are many opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts.

Note

This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!

Best Basic Part Special Prize

To be eligible for this award, this part must adhere to Registry sample submission guidelines and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this special prize, make sure you add your part number to your judging form and delete the box at the top of this page.

Please note: Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize.