Team:NUS Singapore-A/Modelling/Model1

<title> OVERVIEW </title>

Modelling was heavily utilised to obtain a better understanding of our systems, as well as, shaping our experimental designs which helped us save unnecessary wastage of resources and time. We constructed models that allowed us to achieve the following: 1. Preliminary study of our intended biochemical pathway 2. Optimal genetic circuit design 3. Proof that optogenetics can work in our systems and improve upon existing inducible/repressible light systems 4. Simulation and optimisation of our entire experimental process Our MATLAB scripts can be found here.

Our team started with the intention to produce dyes consisting of the primary colours, red, yellow and blue, that can be easily mixed to create a plethora of colours for the textile industry. However, we have decided to produce namely, Chrysanthemin (red) and Luteolin (yellow) instead. This decision was due in part to our interview with Mr Holger Schlaefke (link to our interview here), Global Marketing Manager of DyStar Pte Ltd, that advised us to produce vibrant colours, and how numerous past iGEM teams have attempted to produce Indigo (a colour similar to blue).

PART 1: THE BIOCHEMICAL PATHWAY

Goal of model