Team:Tec-Monterrey/InterLab

Overview

Interlab procedure was made to achieve bronze #4 requirement. To make this, the IGEM 2018 Interlab Study Protocol was followed. To make calibration and experimental data the following material was used: measurement kit which contains Ludox, silica bead and fluorescein, competent cells from E. coli strain DH5a and 96 well plates, black with a clear flat bottom. The first part of the protocol was to make standard curves for OD measurements, cell standard curves and fluorescein standard curves. Using Ludox solution, it was capable to obtain the following reference point for an OD₆₀₀.

Figure 1: Table for the conversion value of OD₆₀₀.

The obtain conversion value obtained is used to convert any measurement of cell density into OD₆₀₀ by multiplying by 2.897 using the same instrument under the same conditions.

Transformation

Absorbance

Fluorescence

Team:Tec-Monterrey/InterLab

Home