Team:Munich/recbiobricks.html

8/31

Assembling biobrick for RecB, C, D

Participants:	eni, katja
Protocol:	PCR, agarose gel, gel extraction, ligation, chem trafo, miniprep
Notes:	RecD_BB_EcoRI_fw & RecD_BB_SpeI_rv (3,5 kb) RecB_BB_EcoRI_fw & RecB_BB_EcoRi_rv (1,8 kb) RecC_BB_EcoRI_fw & RecB_BB_EcoRI_rv (3,3 kb) AT 68° ET 70 s
Results:	PIC only one worked, but somehow all were ligated :D

9/3

Test pcr for Biobricks of RecB, C, D

Participants:	eni
Protocol:	PCR, agarose gel
Notes:	Primers: VF2 & VR
Results:	?