Team:Munich/recbiobricks.html

8/31

Assembling biobrick for RecB, C, D

Participants:	eni, katja
Protocol:	PCR, agarose gel, gel extraction, ligation, chem trafo, miniprep
Notes:	RecD_BB_EcoRI_fw & RecD_BB_SpeI_rv (3,5 kb) RecB_BB_EcoRI_fw & RecB_BB_EcoRi_rv (1,8 kb) RecC_BB_EcoRI_fw & RecB_BB_EcoRI_rv (3,3 kb) AT 68° ET 70 s
Results:	PIC only one worked, but somehow all were ligated :D

9/3

Test pcr for Biobricks of RecB, C, D

Participants:	eni
Protocol:	PCR, agarose gel
Notes:	Primers: VF2 & VR
Results:	?

9/3

Removing PstI restriction site from pSB1C3_RecB

Participants:	eni
Protocol:	PCR, PCR purification, Ligation, gibson assembly, trafo
Notes:	because of time restraints, this experiment was done using ligation and gibson assembly approaches in parallel PCR: RecB_BB_PstI-remove-Lig Primer: RecB_mut_Lig_fw & RecB_mut_Lig_rv Templ: RecB_BioBrick Plasmid AT: 72° ET: 11 min PCR: RecBCD_BB_PstI_remove_lig Primer: RecB_mut_Lig_fw & RecB_mut_Lig_rv Templ: RecBCD_BioBrick Plasmid AT: 72° ET: 11 min PCR: RecBCD-TEV_BB_PstI_remove-Lig Primer: RecB_mut_Lig_fw & RecB_mut_Lig_rv Templ: RecBCD-TEV_BioBrick Plasmid AT: 72° ET: 11 min PCR: RecB_BB_PstI-remove-GA Primer: RecB_mut_fw & RecB_mut_rv Templ: RecB_BioBrick Plasmid AT: 72° ET: 11 min PCR: RecBCD_BB_PstI_remove_GA Primer: RecB_mut_fw & RecB_mut_rv Templ: RecBCD_BioBrick Plasmid AT: 72° ET: 11 min PCR: RecBCD-TEV_BB_PstI_remove-GA Primer: RecB_mut_fw & RecB_mut_rv Templ: RecBCD-TEV_BioBrick Plasmid AT: 72° ET: 11 min
Results:	?