Team:Munich/growth.html

Contents

1 8/28
- 1.1 growth of msbB and Recombinase deficient E.Coli mutant strains
2 8/31
- 2.1 preparing electro-competent cells of WT RecBCD und dRecB
3 9/3
- 3.1 Transforming E.Coli dRecB with pSB1C3_RecBCD(WT)
4 9/4
- 4.1 DNA midi prep of RecBCD(WT) plasmid from Dh5a
5 9/14
- 5.1 growth of RecB, RecD, RecBCD, msbb deficient E.Coli strains

8/28

growth of msbB and Recombinase deficient E.Coli mutant strains

Participants:	katja
Protocol:	Measuring optical density
Notes:
Results:	PIC

8/31

preparing electro-competent cells of WT RecBCD und dRecB

Participants:	katja
Protocol:	preparing electro-competent cells
Results:	cells are still viable after preparation, dRecB grows significantly slower than others

9/3

Transforming E.Coli dRecB with pSB1C3_RecBCD(WT)

Participants:	katja
Protocol:	epo trafo
Notes:
Results:	colonies

9/4

DNA midi prep of RecBCD(WT) plasmid from Dh5a

Participants:	katja
Protocol:	DNA Midi prep
Notes:
Results:	concentration

9/14

growth of RecB, RecD, RecBCD, msbb deficient E.Coli strains

Participants:	katja
Protocol:	Measuring optical density
Notes:
Results:	PIC