Team:Munich/chiversions.html
2018/05/28 - 2018/05/29
Contents
PCR amplify linear mtq2
| Participants: | Dominic |
| Protocol: | PCR, agarose gel, gel purification |
| Notes: | VR & VF2; TA: 66° ET: 50s; Template: linear mtq2_bivtat from Lukas Aufinger (Supervisor) |
| Results: | worked (PIC) |
2018/06/04 - 2018/06/08
assembling Chi6(double) and Chi6-Mtq2-Chi6
| Participants: | Dominic |
| Protocol: | PCR, Agarose gel, gel purification, gibson assembly |
| Notes: | Fragments were amplified, then purified and gibson assembly was performed |
| Results: | No bands were visible on the gel after gibson assembly, suggesting that fragments were not amplified correctly before. additionally, we decided to do overlap pcr instead of gibson assembly. |
2018/06/11 - 2018/06/14
assembling Chi6(double) and Chi6-Mtq2-Chi6
| Participants: | Dominic |
| Protocol: | PCR, agarose gel, gel extraction, |
| Notes: | Fragments were amplified, then purified and overlap PCR was performed. |
| Results: | The Agarose gel after overlap PCR was produced incorrectly, therefore we lost the samples. |
2018/06/12
PCR to amplify Mtq2
| Participants: | Dominic |
| Protocol: | PCR |
| Notes: | Primers: VF2, VR |
| Results: |