Team:Munich/deletioncasette.html
Transforming E. Coli DH5a to amplify pKD3 for pRED/ET engineering
2018/05/28| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | we also inoculated LB media blindly. |
| Results: | no colonies, no growth; We decided to use a resistance cassette from pSB1C3 without FRT sites |
Amplifying a selection cassette from pSB1C3
2018/05/29| Participants: | Eni |
| Protocol: | PCR, agarose gel, gelextraction |
| Notes: | Primer with 50bp homology regions ????, TA: 67°C, Elongation: 45s |
| Results: | PIC. we thought the band might be what we wanted but because of how few DNA the extraction yielded, these samples were not used in further experiments. |