Team:Munich/engineering2.html
Contents
Transforming E. Coli Rosetta and MG1655 for pRED/ET engineering
2018/08/28
| Participants: | Dominic |
| Protocol: | epo trafo |
| Notes: | MG1655 is E.Coli WT as backup |
| Results: | colonies only on MG1655 plate
No colonies on E.coli Rosetta |
8/29
pRED/ET genome engineering of delta msb-B and delta recBCD strains
| Participants: | Dominic |
| Protocol: | pRED/ET engineering protocol |
| Notes: | the template for the resistance cassette for deletions was taken from a plasmid containing mRFP |
| Results: | red colonies
Redoing the experiment but doing a dpni digest before |
8/30
Transforming E. Coli DH5a to find a reason for the contamination
| Participants: | Dominic |
| Protocol: | epo trafo |
| Notes: | CAP_recBCD, CAP_msbb, psb1c3_mrfp in Dh5a |
| Results: | red colonies on all plates -> mRFP contamination |