Team:Munich/engineering2.html
Transforming E. Coli Rosetta and MG1655 for pRED/ET engineering
2018/08/28
| Participants: | Dominic |
| Protocol: | epo trafo |
| Notes: | Genetic engineering is planned in E. Coli Rosetta, MG1655 is E.Coli WT as backup. |
| Results: | colonies only on E. Coli MG1655 plate;
No colonies on E. Coli Rosetta |
pRED/ET genome engineering of delta msb-B and delta recBCD strains
2018/08/29
| Participants: | Dominic |
| Protocol: | pRED/ET engineering protocol |
| Notes: | the template for the resistance cassette for deletions was taken from a plasmid containing mRFP |
| Results: | red colonies. Elena (Advisor) and Dominic decided to repeat the experiment but to do a dpnI digest before to get rid of the template DNA. |
Transforming E. Coli DH5a to find a reason for the contamination
2018/08/30
| Participants: | Dominic |
| Protocol: | epo trafo |
| Notes: | CAP_recBCD, CAP_msbb, psb1c3_mrfp in Dh5a |
| Results: | red colonies on all plates -> mRFP contamination |