Team:Munich/engineering4.html

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Transforming E. Coli Rosetta for pRED/ET engineering

2018/09/05
Participants: Dominic
Protocol: epo. Trafo
Notes: we used 1µg of DNA for maximum efficiency
Results: No colonies. Something prevents E.Coli Rosetta from being transformed with pRED/ET. Ori's have been checked for compatibility and E.Coli Rosetta can be transformed with plasmids otherwise. We switched focus from this part of the project which is why we did not continue on those results.

DNA preparation for pRED/ET Engineering

2018/09/05
Participants: Dominic
Protocol: mini prep
Notes:
Results: 48ng/ul; This sample was prepared for storage in the Simmel Lab.