Team:CPU CHINA/Notebook/Log
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Early Stage
As is shown in Human Practice (Silver), we China Pharmaceutical University has a club for synthetic biology and iGEM. This year’s team started from Kexin Cao, Yunge Lou, Junqing Zhang, Shuyao Zhou and Huandi Xu. These 5 have long been club members of CPU Bio-X Club.They witnessed the success of their senior schoolmates, and was determined and ready for this year’s competition. After intense group study and brainstorming for 4 months, Shuyao Zhou’s scheme of condition RNA interference (though very rough compared with today’s whole project) was the most feasible one, and was approved by our PI Prof. Tian.
12/2-12/9/2017
Site-directed mutagenesis of hTERT and HULC promoter sequences.(Design) Contemplating on what (mathematical) model would apply.(Model) Literature research: the significance of MAP4K4 gene and how the tumor would react after it is silenced, how the inhibitory strand could be transcripted.(Design) Write an experiment protocol: subcutaneous tumorigenesis in nude mice.(Lab) Select a proper RdRP.(Design)
12/10-12/12/2017
Select aligand-inducible promoter/RdRP inhibitors.(Design) Determine the sequence of hTERT and HULC promoter.(Design) Literature research: the functioning mechanism of MAP4K4 in hepatocellular carcinoma.(Design) Analytical method of HCV RdRP.(Lab) Design a double-ncRNA-encoding vector.(Design) Select RdRP and ncRNA sequence.(Design)
1/21-1/26/2018
Design a miRNA spongewhich couldtarget Sil-MAP4K4.(Design) Research on vectors.(Design) Construct CMV-tet-tTA system.(Design) Design the pSil-MAP4K4 and inhibitory strand.(Design) Write aproposal about the whole scheme, including the principle, technical route and research contents, for our PI to systematically evaluate.(Design)
1/27-1/28/2018
Make a budget for experimental materials and reagents.(Lab)
1/29-1/31/2018
Plan the whole experimenting process.(Lab) Prepare a schematic diagram.(Design)
Winter vacation
Sign up and pay the fees.
3/4-3/9/2018
Determine the exact sequence of the miRNA sponge.(Design) Combine the HULC promoter and pSil-MAP4K4 sequence.(Design) Call for the synthesisof parts involved in Plasmid 1 by biotechnology company.(Experiment)
3/10-3/20/2018
Determine the exact sequence for parts involved inPlasmid 2.(Design) Brainstorming a brand name for ourproject.(Miscellaneous) Contemplating on what (mathematical) model couldbe applied to non-experimental use in the whole project. (Model) Recruit new members. Nannan Liu and Wanli Zhang joinedour team!
3/21-3/26/2018
Hongyu Wang, Yiyang Zhu, Peng Sun and Ying Miao joined us! Ponder the novelty of the project.(Design)
3/27-4/1/2018
Design a scheme for Human Practice.(HP)
4/1-4/7/2018
Determine the project's name.(Miscellaneous) Prepare for cross-school talks.(HP) Finish the presentation for the funding from“Training Program of Innovation and Entrepreneurship of College Students in China Pharmaceutical University”.(HP) Research on the general evaluation method for a cancer treatment protocol.(HP)
4/14-4/22/2018
Investigate the pharmacology property of two first-line treatment macromolecular drugs, both delivered by lipidosome.(HP) Design the team uniform, working card, team logo and a presentation scroll.(Miscellaneous) Look for sponsorship.(Sponsor)
4/23-4/28/2018
Draw up a plan of interviewing experts in different but relative fields.(HP) Validate the model structure and design a questionnaire about the costs and side reaction of therapies for HCC.(Model)
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To be continued.
