Team:Goettingen/Notebook/biobricks April

All basic parts were created via standard cloning techniques and methods are described <a href="https://2018.igem.org/Team:Goettingen/Experiments" target="_blank">here</a>. The composite parts were constructed, using the 3A-assembly, which is also described <a href="https://2018.igem.org/Team:Goettingen/Experiments" target="_blank">here</a>. The following table gives an overview about our parts.

Part	What?	How?	Sequenced?	Ok?
BB1	gltT	CCR, cloning	no mutations	✔
BB2	gltP	PCR, cloning	no mutations	✔
BB3	Palf4	PCR, cloning	no mutations	✔
BB4	RBS	PCR, cloning	no mutations	✔
BB5	RBS-gltT	3A-assembly		✘
BB6	Palf4-RBS	3A-assembly	no mutations	✔
BB7	Palf4-RBS-gltT	3A-assembly	no mutations	✘
BB8	aroE	CCR, cloning	no mutations	✔
BB9	aroA	PCR, cloning	no mutations	✔
BB10	PtrpP	PCR, cloning	no mutations	✔
BB11	gat	PCR, cloning	no mutations	✔
BB12	aroA*	PCR, cloning	no mutations	✔
BB13	Palf4-RBS-gat	3A-assembly		✘
BB14	Palf4-RBS-aroA*	3A-assembly		✘
BB15	Pcomm	not yet		✘
BB16	Strep-tag-aroE	PCR, cloning		✘
BB17	Strep-tag-aroA	PCR, cloning		✘
BB18	PyciC–xylR	3A-assembly		✘
BB19	PyciAB–xylR	3A-assembly		✘
BB20	PyciC	PCR, cloning		✘
BB21	PyciAB	PCR, cloning		✘
BB22	xylR	PCR, assembly		✘