All basic parts were created via standard cloning techniques and methods are described <a href="https://2018.igem.org/Team:Goettingen/Experiments" target="_blank">here</a>. The composite parts were constructed, using the 3A-assembly, which is also described <a href="https://2018.igem.org/Team:Goettingen/Experiments" target="_blank">here</a>. The following table gives an overview about our parts.
Part | What? | How? | Sequenced? | Ok? |
---|---|---|---|---|
BB1 | gltT | CCR, cloning | no mutations | ✔ |
BB2 | gltP | PCR, cloning | no mutations | ✔ |
BB3 | Palf4 | PCR, cloning | no mutations | ✔ |
BB4 | RBS | PCR, cloning | no mutations | ✔ |
BB5 | RBS-gltT | 3A-assembly | ✘ | |
BB6 | Palf4-RBS | 3A-assembly | no mutations | ✔ |
BB7 | Palf4-RBS-gltT | 3A-assembly | no mutations | ✘ |
BB8 | aroE | CCR, cloning | no mutations | ✔ |
BB9 | aroA | PCR, cloning | no mutations | ✔ |
BB10 | PtrpP | PCR, cloning | no mutations | ✔ |
BB11 | gat | PCR, cloning | no mutations | ✔ |
BB12 | aroA* | PCR, cloning | no mutations | ✔ |
BB13 | Palf4-RBS-gat | 3A-assembly | ✘ | |
BB14 | Palf4-RBS-aroA* | 3A-assembly | ✘ | |
BB15 | Pcomm | not yet | ✘ | |
BB16 | Strep-tag-aroE | PCR, cloning | ✘ | |
BB17 | Strep-tag-aroA | PCR, cloning | ✘ | |
BB18 | PyciC–xylR | 3A-assembly | ✘ | |
BB19 | PyciAB–xylR | 3A-assembly | ✘ | |
BB20 | PyciC | PCR, cloning | ✘ | |
BB21 | PyciAB | PCR, cloning | ✘ | |
BB22 | xylR | PCR, assembly | ✘ |