Team:Goettingen/Notebook/biobricks August

All basic parts were created via standard cloning techniques and methods are described <a href="https://2018.igem.org/Team:Goettingen/Experiments" target="_blank">here</a>. The composite parts were constructed, using the 3A-assembly, which is also described <a href="https://2018.igem.org/Team:Goettingen/Experiments" target="_blank">here</a>. The following table gives an overview about our parts.

Part What? How? Sequenced? Ok?
BB1 gltT CCR, cloning no mutations
BB2 gltP PCR, cloning no mutations
BB3 Palf4 PCR, cloning no mutations
BB4 RBS PCR, cloning no mutations
BB5 RBS-gltT 3A-assembly
BB6 Palf4-RBS 3A-assembly no mutations
BB7 Palf4-RBS-gltT 3A-assembly no mutations
BB8 aroE CCR, cloning no mutations
BB9 aroA PCR, cloning no mutations
BB10 PtrpP PCR, cloning no mutations
BB11 gat PCR, cloning no mutations
BB12 aroA* PCR, cloning no mutations
BB13 Palf4-RBS-gat 3A-assembly
BB14 Palf4-RBS-aroA* 3A-assembly
BB15 Pcomm not yet
BB16 Strep-tag-aroE PCR, cloning
BB17 Strep-tag-aroA PCR, cloning
BB18 PyciC–xylR 3A-assembly
BB19 PyciAB–xylR 3A-assembly
BB20 PyciC PCR, cloning
BB21 PyciAB PCR, cloning
BB22 xylR PCR, assembly