Team:Goettingen/Notebook/biobricks September

All basic parts were created via standard cloning techniques and methods are described <a href="https://2018.igem.org/Team:Goettingen/Experiments" target="_blank">here</a>. The composite parts were constructed, using the 3A-assembly, which is also described <a href="https://2018.igem.org/Team:Goettingen/Experiments" target="_blank">here</a>. The following table gives an overview about our parts.

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Part	What?	How?	Sequenced?	Ok?
BB1	gltT	CCR, cloning	no mutations	✔
BB2	gltP	PCR, cloning	no mutations	✔
BB3	Palf4	PCR, cloning	no mutations	✔
BB4	RBS	PCR, cloning	no mutations	✔
BB5	RBS-gltT	3A-assembly	no mutations	✔
BB6	Palf4-RBS	3A-assembly	no mutations	✔
BB7	Palf4-RBS-gltT	3A-assembly	no mutations	✘
BB8	aroE	CCR, cloning	no mutations	✔
BB9	aroA	PCR, cloning	no mutations	✔
BB10	PtrpP	PCR, cloning	no mutations	✔
BB11	gat	PCR, cloning	no mutations	✔
BB12	aroA*	PCR, cloning	no mutations	✔